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Anew study by Schenkman et al. on page 829 reveals that the subcellular localization of yeast budding proteins can be determined by the time the genes are expressed.
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The genes BUD8 and BUD9 are required for establishment of the bipolar budding pattern in normal diploid cells, in which both poles are specified as potential bud sites. Although the two proteins are similar in structure, they show distinct subcellular localizations, consistent with functions as markers of the distal and proximal poles of daughter cells.Using promoter swap experiments, Schenkman et al. now show that the timing of gene expression determines the localization of Bud8p and Bud9p. The localization of the proteins is probably determined by the direction of the general secretory vesicle traffic at the stage of the cell cycle when the protein is delivered. Although new Bud8p is delivered shortly before or concurrent with bud emergence, and thus will be found at the distal end of the bud, new Bud9p is deposited to the bud side of the neck very late in the cell cycle, and is thus found at the proximal pole of new daughter cells.
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The group's analysis also determined that the peak in transcription of the BUD8 and BUD9 mRNAs precedes the delivery of the corresponding proteins to the cell surface by about an hour. This could be accomplished by a delay in either the translation of the protein or the trafficking of the protein to the cell surface.(The location of budding proteins is cont) |
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发表于 2009-3-6 00:15
发表于 2026-10-3 11:58
