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本帖最后由 细胞海洋 于 2012-8-27 23:23 编辑 ) v, |4 N t/ S, z( U) V
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Edited by- |5 I8 c2 n5 d. J- {
Uma Lakshmipathy
I3 E, S, s) NBhaskar Thyagarajan* k, O0 Q% Y) Y4 z! C; t
CONTRIBUTORS xi
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7 P2 N9 o( u8 Z( h) B4 P% dPART I: CLONING AND GENE DELIVERY 19 K; h, [- \+ r3 c) x- u3 I1 A" c0 y
1. DNA Assembly Technologies Based on Homologous6 ~ J* R, R9 D2 i6 j0 M
Recombination 3
( L9 `# b' t/ S5 L1 hBillyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang,+ K! O& v5 P+ Y
Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki,
3 ` `5 m% A7 E9 M+ s: PTodd Peterson, and Federico Katzen
" j( z7 K* Y$ Q. o2 t2 |- {2. Multigene Assembly for Construction of Synthetic Operons:
R6 B, P+ p& g1 wCreation and Delivery of an Optimized All-IN-One
0 f% P5 m7 B4 T( W5 OExpression Construct for Generating Mouse iPS Cells 19
+ `( L2 q! r n$ j+ F3 U. zTakefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh,/ \* _0 D* x% @1 s6 U2 ]4 v
Fumio Imamoto, and Jonathan D. Chesnut
( O6 ^; v |% }) F+ V3. Strategies for the Delivery of Naked DNA 374 s' M- d8 N ?% }2 h9 l6 E. P
Jennifer C. Moore and Ronald P. Hart" K. i2 W* L9 {( | d* Q
PART II: NONINTEGRATING TECHNOLOGIES 49
0 \! Z6 ]/ U+ A2 s6 F4. Episomal Vectors 51) U' d" F1 a w( _ s
Junying Yu
9 z9 r! R. {' x1 A5 x' d4 ]7 b8 ^5. Nonintegrating DNA Virus 85[attach]45477
4 }& j4 g+ R4 }& }6. Nonintegrating RNA Viruses 103; i* p1 p9 Y. C. J
Noemi Fusaki( l) w" `+ G% E8 o/ W$ T
7. Protein Delivery 1197 ^, O4 O5 w8 L
Xiao-Jian Han and Kazuhito Tomizawa
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; p) W% u1 H8 o7 nPART III: INTEGRATING TECHNOLOGIES 135
# E" M% n- D& J& I: k% Q" q" D& d8. Sleeping Beauty Transposon-Mediated Stable Gene: V9 V+ P$ [6 ?: q
Delivery 137
+ Q( q; C5 s) @* {% a3 wAndrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman,- y3 b ^3 a& n$ T0 P/ d4 R) q
and R. Scott McIvor
' L @7 l$ p1 G; {5 @- O9. Integrating Viral Vectors for Gene Modifications 163
/ b4 I" [4 ~# M. _+ T8 rGemma L. Mendel, Brian Paszkiet, and Rafal P. Witek
, }" E' [( n8 i0 ?" z j# [10. Bacteriophage Integrases for Site-Specific Integration 199
7 t1 \! [0 S% ^( c+ i sBhaskar Thyagarajan
1 e5 @! K. E0 U! o# Q11. Improving Gene Targeting Efficiency in Human
* V, q4 Y3 w- O+ x+ b3 N9 [/ SPluripotent Stem Cells 211, j9 I2 F9 C0 K0 L* ^: P5 y/ a$ T
Sangyoon Han, Anna McCann, Louise C. Laurent,
. ~3 G9 h% B( g$ XJeanne F. Loring, and Ying Liu$ A+ i3 s1 O* T# M; @) A3 a( B4 \
PART IV: APPLICATIONS 227
6 I$ E/ t5 s# E$ h5 c: h12. Modified Stem Cells as Disease Models and in Toxicology( U# a; w5 E. V0 t
Screening 2290 F( J4 o1 f( c/ A. [* q
Catharina Ellerstr
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