The cultures were next supplemented with 20 mg/ml pifithrin- and cultured for 3-4 weeks with unconditioned KOSR + 100 ng/ml b-FGF and week 2-4 with MEF conditioned KOSR + 100 ng/ml b-FGF (Invitrogen)) under low oxygen (2%) conditions with daily medium changes. ! T# _5 ^- V1 z5 C6 o5 O这个condition 和uncondition，就是有没有MEF细胞而定的啊？取细胞分泌的上清去培养别的细胞叫不叫condition的？
发表于 2012-12-25 12:54
发表于 2012-12-27 08:59
