PDF电子书：NanoCellBiology of Secretion

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1 r% {. Y" C2 e4 oNanoCellBiology of Secretion
9 X+ @& C0 @+ S- `0 n9 yImaging Its Cellular and Molecular Underpinnings) R4 v! P3 Z8 V$ l1 l" g
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NanoCellBiology of Secretion ....................................................................... 1  [- c" E4 ]+ l* d! f
Introduction ...................................................................................................... 1/ y: u) s$ k2 t9 \' E9 a  j2 }
Materials and Methods ..................................................................................... 57 }1 c- N, x4 H$ v$ t% a
Pancreatic Acini Isolation ............................................................................ 5; I; N7 c; j! H# J
Zymogen Granule Isolation ......................................................................... 5/ w4 ?1 w6 ?) c- \5 ?/ R
Isolation of Synaptosomes, Synaptosomal Membrane,
- J4 n* Z- L: A& K5 Z+ R) \! aand Synaptic Vesicles ................................................................................... 6
4 b1 [9 V8 Y4 B6 d& IPorosome Isolation ....................................................................................... 6
. k0 p% i1 f% ^$ `+ n# _4 I# wAtomic Force Microscopy ........................................................................... 77 t7 c5 s: _* l. R' y7 o( B) Y" s" L
Measurement of Secretion ........................................................................... 7& x  w, n. N( Z
Transmission Electron Microscopy.............................................................. 8
8 Z% ~0 E) C, x( kDynamic Light Scattering (DLS) to Determine Vesicle
$ w  g# V  O; }4 _9 JVolume Change ............................................................................................ 8
( s0 T7 L, {  }: _  q5 [4 q; M2 N# \Photon Correlation Spectroscopy ................................................................ 8
3 w! }* q0 u8 XPorosome Functional Reconstitution Assay ................................................ 9
7 I! G2 S# v' x& r; v4 v, SPreparation of Liposomes and SNARE Reconstitutions .............................. 9$ L% Y, r" U7 @" k
Circular Dichroism Spectroscopy ................................................................ 10; D, ]- h* @( x5 A+ j
Wide-Angle X-ray Diffraction ..................................................................... 10! L1 C7 q3 o' w7 f9 W+ Y
Porosome Discovery ........................................................................................ 11
6 c0 r, A0 p+ k6 zCalcium and SNARE-Induced Membrane Fusion ........................................... 23
% a' `# d  T: [; Fv- and t-SNAREs Need to Reside in Opposing Membrane# Z, o3 a! \* H$ X
for Appropriate Interactions and the Establishment- q8 e0 R+ J; ^8 @4 x* l
of Continuity Between Opposing Membranes ............................................. 26
4 Z- l# h3 ?" F9 p4 I7 P, JMembrane Curvature Dictates the Size of the SNARE4 X* R& N$ c# `3 t- X+ ]
Ring Complex .............................................................................................. 313 Z9 y  i. ^6 b9 y
Disassembly of the t-/v-SNARE Complex .................................................. 32
4 G; I- x) W, |. `; BCD Spectroscopy Confi rms Membrane Requirement) T) S# e* X8 T" ~8 g  t
for Appropriate t-/v-SNARE Assembly and Suffi ciency
3 G* T% U% N+ ?of NSF–ATP for Its Disassembly ................................................................. 38
6 \6 P5 C: s) V! r: S+ t/ A. YSNAREs Bring Opposing Bilayers into Close Approximation,! q( Z1 d, I" H# Y, `! U  F
Enabling Calcium Bridging and Membrane Fusion .................................... 407 J  O1 }/ O0 p
Membrane Lipids Infl uence SNARE Complex
  f4 I6 ^+ \6 R6 S) [5 c; iand Assembly–Disassembly ........................................................................ 42
7 J. d$ K# q& iSecretion Involves Vesicle Swelling and Content Expulsion........................... 47' D3 U% V9 W$ W  Z: y; X
Secretory Vesicle Swelling Is Required for the Expulsion1 @  d9 s1 |! U3 W
of Intravesicular Contents During Cell Secretion ........................................ 47- [3 V. q% f1 x# G: r: X
Molecular Mechanism of Secretory Vesicle Swelling ................................. 53
0 W/ Z0 k8 @( I0 s/ z8 xPresence of Functional b-Adrenergic Receptors at the Synaptic8 ~7 V8 c& M% w' D" q0 ~& j% V
Vesicle Membrane ........................................................................................ 58, y, k+ W$ R2 [) c$ I
Conclusion ....................................................................................................... 62. r6 x4 q7 b) B; p
References ........................................................................................................ 63
1 n. m, i3 |8 n" Q2 y- R3 SIndex ................................................................................................................ 69; e) m& l, P  i+ Z: g# c
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kouke80

好书。谢谢。

laoli1999

支持一下吧  

yukun

呵呵 高高实在是高~~~~~  

aakkaa

回帖是种美德.  

marysyq

不错 不错  比我强多了  

dypnr

鉴定完毕.!  

beautylive

我该不会是最后一个顶的吧  

陈晴

站个位在说  

依旧随遇而安

(*^__^*) 嘻嘻……