PDF电子书：NanoCellBiology of Secretion

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NanoCellBiology of Secretion4 i2 k  z0 O9 \! F: N, |' u
Imaging Its Cellular and Molecular Underpinnings) s2 `0 V$ D! E) l8 s/ O

" O1 }; F+ `0 ^; e2 }NanoCellBiology of Secretion ....................................................................... 1: y6 b: T/ w+ x$ y; s# W
Introduction ...................................................................................................... 1
( P2 r7 C  `1 d! SMaterials and Methods ..................................................................................... 50 G( p& S, l2 T, B6 I! q7 J
Pancreatic Acini Isolation ............................................................................ 51 g" z1 ?: S' n$ y3 J' D
Zymogen Granule Isolation ......................................................................... 5
( |7 V( l  Y3 F' T  LIsolation of Synaptosomes, Synaptosomal Membrane,: w4 x) [3 p) g& _
and Synaptic Vesicles ................................................................................... 6
4 N3 K/ s% ~5 M6 vPorosome Isolation ....................................................................................... 6
) G# Q) p7 d: d( N: [6 v* O2 vAtomic Force Microscopy ........................................................................... 7
0 B8 a" z9 a: ~Measurement of Secretion ........................................................................... 7( O& g. s; A3 ?( y9 h
Transmission Electron Microscopy.............................................................. 8
% i- }4 R" d$ K  aDynamic Light Scattering (DLS) to Determine Vesicle
6 Z+ L' Q& _/ f0 J4 uVolume Change ............................................................................................ 82 Y- E0 |) l1 X& z: P: _8 k" [  Q
Photon Correlation Spectroscopy ................................................................ 8
) S0 x8 ?$ k3 E0 S" B9 V7 P+ RPorosome Functional Reconstitution Assay ................................................ 9
4 D( t3 C( E) h2 E5 ?5 d% U2 MPreparation of Liposomes and SNARE Reconstitutions .............................. 9. j* ~3 v# Z0 F1 p3 N; q5 l( e/ R
Circular Dichroism Spectroscopy ................................................................ 10
6 Q4 S8 Y1 Z" q9 SWide-Angle X-ray Diffraction ..................................................................... 105 B) P8 U9 t5 ]  i
Porosome Discovery ........................................................................................ 11. A* @( l, o  Z# y6 t- U
Calcium and SNARE-Induced Membrane Fusion ........................................... 233 r) L# y' W0 h, B0 s
v- and t-SNAREs Need to Reside in Opposing Membrane! F  k) F1 o' ^2 A
for Appropriate Interactions and the Establishment# S0 l3 v: l6 `- t1 s3 g# v
of Continuity Between Opposing Membranes ............................................. 26+ S, R9 E/ V; C
Membrane Curvature Dictates the Size of the SNARE7 p5 ~% T$ P( b# c7 A
Ring Complex .............................................................................................. 31/ |; B) ^  \; t) A
Disassembly of the t-/v-SNARE Complex .................................................. 323 y  o* a/ W$ j1 \0 M. H8 o& h6 \
CD Spectroscopy Confi rms Membrane Requirement# F; z7 k* n/ d
for Appropriate t-/v-SNARE Assembly and Suffi ciency: s, S* c1 _* N+ Y2 V
of NSF–ATP for Its Disassembly ................................................................. 385 Q! V3 C3 B5 ^6 L: k
SNAREs Bring Opposing Bilayers into Close Approximation,
" h- S; m5 v3 J4 ZEnabling Calcium Bridging and Membrane Fusion .................................... 40. }7 y) Z1 q7 b4 o/ i, Y
Membrane Lipids Infl uence SNARE Complex  M/ v* |5 p9 ^$ a6 `
and Assembly–Disassembly ........................................................................ 42
9 a4 o" `2 g- k. jSecretion Involves Vesicle Swelling and Content Expulsion........................... 47# X! k- h5 e& i' n
Secretory Vesicle Swelling Is Required for the Expulsion
: V6 A& Z- Q6 Y$ M' l$ Lof Intravesicular Contents During Cell Secretion ........................................ 47
7 W2 y6 Q3 j+ y$ e) ~Molecular Mechanism of Secretory Vesicle Swelling ................................. 53
3 P. r/ s& L6 n, x7 ]Presence of Functional b-Adrenergic Receptors at the Synaptic
1 u+ ]+ H0 f! {4 aVesicle Membrane ........................................................................................ 58- H2 |4 V, I& h9 j4 W
Conclusion ....................................................................................................... 62- F. t$ \" }$ q1 N: `  k
References ........................................................................................................ 63
  T! }# [0 I7 K9 E. S" f- VIndex ................................................................................................................ 69* `$ x) e# G$ o: q& ?6 N" u
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kouke80

好书。谢谢。

laoli1999

支持一下吧  

yukun

呵呵 高高实在是高~~~~~  

aakkaa

回帖是种美德.  

marysyq

不错 不错  比我强多了  

dypnr

鉴定完毕.!  

beautylive

我该不会是最后一个顶的吧  

陈晴

站个位在说  

依旧随遇而安

(*^__^*) 嘻嘻……