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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑
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《神经生物学实验操作手册》目录
6 K$ ~' d! k! a% w$ G简介 * _! e& h |: O L) X& v7 k8 C
神经干细胞及神经发育 ······································································5
6 W5 B/ Y. J' f# Q. P 神经系统疾病中的神经细胞类型 ···························································8
( @8 C3 c8 t0 w: B2 D神经细胞培养及分化 $ ]3 q/ G: L. ^+ {+ V
人神经干细胞培养 ············································································ 11* u D' h- k( |% b& @
大鼠胚胎神经干细胞培养 ····································································15* f$ k4 u3 [! \! O
神经干细胞的无外源成分培养 ·······························································19
( u- p8 n. \( G; }0 z& K 人多能干细胞诱导成神经干细胞 ····························································21
3 B ~. e# [. z: T 神经干细胞分化为神经元和神经胶质细胞 ················································317 V. R% y$ T6 e( X, ?
神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
+ ^" }. f7 k: F+ e I2 C0 B7 V 皮层星形胶质细胞的诱导和培养 ···························································414 v- M0 E) f6 G, g @
皮层及海马神经元的分离、培养和鉴定 ··················································43
9 E# j& J, f2 I 多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································486 h5 O, _( z8 X! E5 ~6 ~/ {2 A% N
多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································566 l, `6 Y2 M+ e2 [
神经干细胞的冻存 ············································································60
5 P: J6 V$ r9 A8 _! E 冻存及复苏成熟分化的神经细胞 ···························································62: j, s/ n8 Z& L
细胞分析 + }+ ~7 M5 K5 _4 D, J# w7 E; f
神经干细胞的细胞活性分析 ·································································64+ h! X* p( q( \" T7 _
神经亚型特性标志 ············································································66 m" w0 a6 f# s
表面标志流式细胞分析 ······································································67
0 p7 L% U" ]3 a4 @6 a免疫细胞化学分析 ···············································································69
9 i5 u l; v2 A" |+ q# d使用Fluo-4 进行细胞内钙流检测 ······························································72
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发表于 2013-10-18 13:08
发表于 2013-12-3 13:52
书已经收到，赞一个。
能不能给我也发一份
