干细胞之家 - 中国干细胞行业门户第一站

 

 

搜索
朗日生物

免疫细胞治疗专区

欢迎关注干细胞微信公众号

  
查看: 623139|回复: 258
go

A portrait of the nuclear pore complex [复制链接]

Rank: 7Rank: 7Rank: 7

积分
威望
0  
包包
3465  
楼主
发表于 2009-3-6 08:48 |只看该作者 |正序浏览 |打印
They zoomed in on pores〞some still embedded in the nuclear membrane and others that they had broken free〞using Fourier analysis to enhance the electron micrographs and eke out more structural detail (Unwin and Milligan, 1982). The pair identified four, previously unrecognized pore components: thin rings resembling washers; spokes that clustered inside the rings; a central hub, or plug; and large particles that sometimes sat on the pore's cytoplasmic side. These pieces gave the pore its cylindrical shape, the researchers concluded, with the spokes affixing the complex to the membrane.
$ a: c4 j6 Q3 W. A0 e. x! m5 g8 L( N3 U9 _, C4 y* y! `: J+ O6 [
Flanked by rings on the cytoplasmic and nuclear sides, the spokes extend inward toward the hub. What the large particles were and what they were doing was a mystery. The researchers raised the possibility that they were ribosomes〞and took a lot of grief for it, Milligan recalls. He now thinks they could be structures that collapsed during preparation of the membranes. The central plug was also puzzling〞Milligan speculates that it could be material passing through the pore.5 Q! r  j2 o- {, y- R. L# ]
7 J- l( _" V' E' L* D) Z
Nuclear pore complexes include spokes (S), central plugs (C), and rings (R)." ?9 k& @1 S% M2 f* }

6 a& ^: Y6 y2 {0 G' Q% J( c# PUNWIN
9 _4 o% w9 U1 e& m4 }6 g, I5 {* o) H1 i
Subsequent studies added some elaborations to this layout. For example, the spokes are much more complex than previously thought, and their inner portions are connected (Akey, 1989; Hinshaw et al., 1992). Jarnik and Aebi (1991) identified filaments emanating from the rings on both sides of the pore, while Goldberg and Allen (1992) spotted basket-like features hanging from the nuclear side. And by analyzing the proteins in the pore complex, Rout et al. (2000) suggested that it contains no more than 30 components〞less than half as many as a ribosome. But the basic arrangement that Unwin and Milligan deduced proved accurate, Milligan says. "The value of the work," he says, "is that it provides a realistic and detailed picture that one can have in one's head" while thinking about possible models of the pore's transport function.5 {3 c; L; l+ d6 n: `9 y6 Q1 ?

* \) h  R7 }( r, }Akey, C.W. 1989. J. Cell Biol. 109:955–970., k0 c! R. l* X. O8 i7 h& y

/ z9 h. s9 Z- |" l; A+ A& d% @9 }Goldberg, M.W., and T.D. Allen. 1992. J. Cell Biol. 119:1429–1440.
. C+ h+ }6 v- H- q6 r9 b$ l& y. Y& \9 \/ d) }* m5 n7 |. W
Hinshaw, J.E., et al. 1992. Cell. 69:1133–1141.
$ l/ }% n( N. u! H* O1 q, X
  h$ |9 N+ [. s9 ]3 A9 s& W7 U" }Jarnik, M., and U. Aebi. 1991. J. Struct. Biol. 107:291–308.
% ?" e& E+ o" f% H* _- e/ t  y6 a" t. D0 [0 n8 M- K
Rout, M.P., et al. 2000. J. Cell Biol. 148:635–651.: N: N4 \* U0 l& v, d$ \0 P

3 u$ b) O" Z) ?3 C& f! v8 fUnwin, P.N.T., and R.A. Milligan. 1982. J. Cell Biol. 93:63–75.(Astriking photograph in a cell biology t)

Rank: 1

积分
威望
0  
包包
1544  
259
发表于 2025-5-3 14:14 |只看该作者
原来是这样  

Rank: 2

积分
162 
威望
162  
包包
1724  
258
发表于 2025-3-25 05:05 |只看该作者
细胞治疗行业  

Rank: 2

积分
72 
威望
72  
包包
1704  
257
发表于 2025-3-13 07:05 |只看该作者
干细胞之家微信公众号
不错啊! 一个字牛啊!  

Rank: 2

积分
162 
威望
162  
包包
1724  
256
发表于 2025-3-9 12:35 |只看该作者
今天临床的资料更新很多呀

Rank: 2

积分
104 
威望
104  
包包
1739  
255
发表于 2025-2-15 22:53 |只看该作者
原来是这样  

Rank: 2

积分
156 
威望
156  
包包
1861  
254
发表于 2025-1-18 03:01 |只看该作者
牛牛牛牛  

Rank: 2

积分
135 
威望
135  
包包
1750  
253
发表于 2025-1-17 06:18 |只看该作者
帮顶  

Rank: 2

积分
69 
威望
69  
包包
1788  
252
发表于 2025-1-3 08:35 |只看该作者
很有吸引力  

Rank: 2

积分
51 
威望
51  
包包
1711  
251
发表于 2024-12-5 21:57 |只看该作者
干细胞研究人员的天堂
‹ 上一主题|下一主题
你需要登录后才可以回帖 登录 | 注册
验证问答 换一个

Archiver|干细胞之家 ( 吉ICP备2021004615号-3 )

GMT+8, 2025-5-29 19:25

Powered by Discuz! X1.5

© 2001-2010 Comsenz Inc.