哈佛Brigham and Women's hospital公布了STAP试验protocol

tinatina

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We have found this to be an effective protocol for generating STAP cells in our lab, regardless of the cell type being studied. The protocol below is a combination of the two most effective approaches described in our Jan 31, 2014 article published in Nature (Obokata et al., Stimulus Triggered Fate Conversion of Somatic Cells into Pluripotency. Nature 505. 641-647, 2014). It is very important that each step be performed precisely as described. The protocol is extremely simple, but will vary slightly if you are starting with tissue rather than a cell suspension. It also will vary depending upon the cell type or tissue with which you are starting. It is important to not skip any steps. It is especially important to triturate the cell suspension for a minimum of 30 minutes, until the suspension can be easily triturated up and down the reduced bore pipettes of the smallest orifices. We first describe the protocol when starting with a suspension of cells, and then describe additional steps necessary when starting with a soft tissue." k( g5 V& `* `# e# E+ c
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sycdegcb

非常感谢分享

zamaoji

我怎么下载下来的不是Pdf格式的，怎么下，求助中。。。

cuixinger

不要折磨我们了  是真是假 给个痛快的吧

wwtbz

谢谢！

creatine

有大神会真的去重复这个实验么~~求结果~

grassroot

看看，谢谢~

饶冠华

re 看看

yylhxylong

看一看  ~~~

Andyhigh

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