Improved Method for Adenovirus and Lentivirus Purification using the Fast-Trap

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Charles Neville, Janet Smith, and Kathleen Ongena, Ph.D., Millipore Corporation, Danvers, MA4 U; s! i+ o5 y; n" N2 ?8 t

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Highly purified virus is necessary for many viral vector' ]4 W. `4 x1 T1 X
applications such as vaccine production and genetic
8 i& @; d- S& ]. H2 r9 |modification. Crude virus preparations often contain cellular, `( e0 k7 t8 R9 F. y, V
debris and proteins from culture media that are toxic to
/ l/ x, d* K& G4 Starget cells and can cause immunogenic reactions in vivo.
+ C% {% `* e2 K" T4 E0 |" V  xConventional virus purification methods based on sucrose; Z( f7 y5 k6 H: K. a
or cesium gradient ultracentrifugation are time-consuming,1 _% C' j1 r* i: j
difficult, and require access to special instrumentation. These
& |% j* t1 i; y% B; d4 }& T+ Y5 ^4 B, Rmethods also often result in low virus recovery.
- R# t' A/ }. W) i% G- Q7 H, k+ GHere we report a fast and easy method for adenovirus# q( ~& ]+ A6 _6 S! @7 q' t# w" d
and lentivirus purification using the Millipore Fast-Trap™ Virus
# p; X" }& j2 OPurification and Concentration Kit. Both adenovirus (Cat. No.
9 {# c$ P, ~0 P1 y- j) \FTAV 000 03) and lentivirus (Cat. No. FTLV 000 03) kits contain& y2 G, @  R7 ]+ {; B2 J6 C
the necessary reagents and filter devices to accommodate8 D, J% Q8 q- j
the entire virus purification workflow (Figure 1). The5 D- z8 E5 Y- ^( K+ n
purification is membrane-based and utilizes a closed vacuumdriven
) t/ j- x6 f" m/ I0 g5 M. Qdevice. High recoveries of purified viable viral particles
% z0 R: r/ X& l8 k% Pare typically achieved in under two hours.
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2 z- Q. b9 S/ F) R[hide][/hide]

mjfmjf

不知道多少钱？

mda-7

I'm engaged in construction of Ad vectors and annoyed about the purification.  Thx.

tspaulzhao

Thanks.

xuliangytu

thanks

dengyanfei

have a look

maxiaorongqp

我是 来下文件的

abu2008

有这么好试剂盒

ouyi2738

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