Nature Biotechnology 16, 642 - 646 (1998) 1 T! m) D8 k, B" L$ Q; H; q
doi:10.1038/nbt0798-642 ) u5 [3 A9 M6 u* w# Q 0 W0 R9 z/ K( i+ Y" ^: \6 T: U, l: P6 I Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cellsJose B. Cibelli1, ,2, Stevem L. Stice2, Paul J. Golueke2, Jeff J. Kane2, Joseph Jerry1, Cathy Blackwell2, F. Abel Ponce de León2, ,3 & James M. Robl1, ,2, ,*+ B. F! Z6 {1 E5 E s5 C
0 u9 v4 w3 H: [) f' M f 9 A) g8 _! a/ V--------------------------------------------------------------------------------+ X. a6 }+ C2 o$ Q) R/ @8 V, k
! m: D) C% g9 XAbstract We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endo-derm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy. ! t; I6 |8 Z7 P% ^6 M / @( m2 h# Y q* Z 全文链接8 C4 h+ b8 f" ?& ? http://www.nature.com/nbt/journal/v16/n7/abs/nbt0798-642.html作者: inankai 时间: 2011-2-16 09:53
Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells 1 O- h1 {. w6 n7 P9 U, a1998作者: zlx0814 时间: 2011-2-16 11:39