本帖最后由 细胞海洋 于 2014-2-10 21:11 编辑 . u' L- v A7 E, Y* Z1 J! i( E5 U ~$ |) W6 h" l( j* i8 \3 ZCurrent Protocols in Stem Cell Biology 2007年完整版 1451页* A* M2 e8 }: j% k
; p) Z, R: U6 `3 E% z9 b e
Online ISBN:9780470151808. A" l- C; l- X1 a- f; h0 ?
DOI: 10.1002/9780470151808 2 G3 B8 S3 t: m8 T* dTable of Contents3 t2 a4 r# M) w4 }3 @
1. Foreword7 b- z$ x- g! A& S% ]6 v; J
2. Preface 7 t/ H! j! G1 `# s G) B3. Chapter 1 Embryonic and Extraembryonic Stem Cells" f4 p2 l+ T+ y7 S6 M ~) P
1. Section A Isolation of Embryonic Stem Cells & A' u& H6 [3 s/ W7 }( Z+ V( o1. Introduction! }( k) t, m4 W+ u9 z5 i. P
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells) {2 J8 }8 o" `- K7 _
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts " O% a. e4 i: x6 }( Q0 Z4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells0 u2 O% c9 m C( Z& L+ G
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment4 K% I4 j2 D. S! r0 v) N, D: D* m
2. Section B Characterization of Embryonic Stem Cells % |/ }/ ?3 ^: u1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells * v$ _9 i& f8 r2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and 6 R d4 R/ f' u% ^Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform9 h% s8 s6 M' t: A# s
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells! u% U \, J! S' j
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of0 t; w- L+ s' h8 Z3 I1 I: \
Pluripotency9 e0 x0 R2 \9 ~$ }( ]2 F2 {
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells T* K( E! E* O6 _3 `$ i; z8 [0 T
Using In Vivo Biotinylation ! a* F+ r+ C. z8 @" v* F6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem 6 ?9 j7 S9 k/ r* V2 ACells 1 N* M% X& R: m- @5 B7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional, g% b9 I" v; o. D$ E2 V
Profiling9 v/ Z5 C& b$ T1 r/ S+ N
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells ) a0 M0 ]4 S# P. C+ R# H3 I1. Introduction , L6 H$ |" X. A5 e8 ^: P! z) }6 d) g2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro. V( n* T; }& @
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture # P: m w5 n$ b, f4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of4 K2 o6 G% u J" k1 F/ ?% h
Mouse Embryonic Feeder Layer Cells ' x5 ?5 v: l# a, F% A' Q# T% J5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells" k% _5 F8 l2 _' v0 W5 o9 w& E
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic0 T6 L3 C" D# k; L
Stem Cells7 ~* s: V; @" K+ j4 y/ j
7. Unit 1C.6 Isolation of Human Placental Fibroblasts+ H$ [) i! e. o" d- w8 Q- y
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic 1 |0 o, l: B' J& M' m' B% X# {Stem Cells6 T4 p9 Q- b" M, I+ {% a n
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of @* w1 i; y6 M" `; lROCK Inhibitor " I/ s6 Q# x2 G" d10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells . J* M. R+ P7 h/ u7 T11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent 1 F5 z3 O6 B9 s- X& S0 Y; XStem Cells on Mouse Embryonic Fibroblast Feeder Cells& s2 v- c- `6 t- H4 ]
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers( l1 n S8 X0 E1 E
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells- m8 L: E2 N* }( S6 z
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap/ c l# Q) Y5 u9 U: }/ N3 g5 d
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by. r7 {3 c* c) N& Z9 b
Suspension and Hanging Drop Cultures 0 ?. e1 f, D% ]4 G3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies7 e+ f/ s# U$ P1 }- @9 T$ D
and a Description of the Hematopoietic Blast Colony Forming Assay 1 T+ a6 ^0 X/ a4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically# g9 o X# o/ }9 E2 X& {( X3 E# q
Defined Culture Conditions Y" ?/ K. a& w* a& \- j
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells h. j. P1 O: T- s( J5. Section E Extraembryonic Lineages! f! {" ?$ u( T. e0 X4 X1 ` N# ]
1. Introduction & r" C* y# [3 s9 n2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation8 n5 D6 j/ [2 Q* ?1 s
into Hepatocyte-Like Cells 2 A+ c7 n# t) ~ E% P3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta - k1 A: [% |0 I% \$ j2 C& I4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells* [0 r( L2 y! P |7 f
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells2 n7 M# P( p5 ]& `* U
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells* G7 I0 b& l8 c) `
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use 9 e+ C0 w* V# {5 S S& ^! N) [' ?6. Section F Mesodermal Lineages - N5 \, J& D% L) p6 B. B1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells 4 N! T% t! l" R6 |' a& @1 z G2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture4 ]: B& l- T1 S: A: Q
with Endoderm in Serum-Free Medium4 q7 ~$ v' F8 l, t& z8 ^7 E
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells . ?: U9 O( @6 Y0 }# T6 a* p% _4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood ) S3 x7 ^6 j- R- h: a5 g+ Z5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells 5 E! N q0 Z: n5 S1 a+ R9 b6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation4 \1 W, t+ H8 D
with Stromal Layers 1 i4 \0 r( {. @, s5 C7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary " z; H( {) o, Q! G. UMurine Hematopoietic Progenitors 6 c6 U7 j7 {3 g) I9 P, A8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from 1 v# A% e$ c" V; c" mHuman Embryonic Stem Cells ( x' X) B$ E- O5 Y n$ R9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells ! [7 u- s; b7 H g4 R' ~4 h( B3 Y/ B10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac 7 ]7 l ]3 z" C9 H" l! CProgenitor Cells . G# U% ~9 R+ e2 ~0 n11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the( B" h& s/ ^6 C# K0 e
Hanging-Drop and Mass Culture Methods + ^ d7 n! I% e5 A0 k7. Section G Endodermal Lineages q* o4 v; ~8 R5 D8 B( ]& u$ `- t5 ?
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells # |' r Q' `% b6 n' T. W2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells ' |3 J" Q$ j2 q8 p3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm & q5 T& x# N& }# {8. Section H Ectodermal Lineages8 k6 ~# d7 ~$ J- K6 h
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons& L1 L8 j b1 u. X( S
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by( F. W( S* i" g' M" q2 i
Two-Photon Microscopy - w2 p4 n4 H" j( P7 B4. Chapter 2 Somatic Stem Cells& V0 K( k( K% Z; e/ v; {
1. Section A Hematopoietic Stem Cells: f) U- o! m1 C8 v$ l: }3 L1 @% W2 m
1. Introduction " H: \. I( w* l2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density 4 _; @( X9 p: j8 \+ a- s: sGradient & R R" H5 U4 C8 \4 c3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood " T2 j( i. Y' k1 E! ?4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood l1 Y H$ n0 L6 }
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells7 j" @+ Q0 ?! P4 h* y
from Adult Mouse Bone Marrow ' F. A& _% s* f+ T6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche" W% x* L, S% O. g! K* H
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine# }$ q* p4 e/ U
Embryonic Tissues# z* }; N# F) A7 k. A. d7 Z
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells+ R u8 Q5 N7 j* u( E
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells % E0 Y; `" U: z l) @. M$ k10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta# H4 q1 e, @7 e( l9 N/ X* O
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells # C- S" r* z# K3 S1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human. k1 T% _: F( a1 A. r# N
Tissues ! ~5 o0 ^& ], n, r4 Q2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells: i4 ~ Y+ u- s8 o; d- z5 t
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells % ]9 @. [" I$ a, R: ?3. Section C Cardiovascular Stem Cells " V& Q: {! m* I% m$ h% N1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood $ q, q8 Y4 O2 z# v8 _2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium) u4 L5 D6 K: V4 [3 x1 D5 {
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells! Z" s) A) x+ k+ K' r
4. Section D Neural Stem Cells: n1 X3 Y4 s6 `2 L7 Y- w- ?
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains2 r6 i# Q! U) g- a l2 E
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells! T" u, H; I! {- G9 N2 J, Z
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and ) ?" A. ?) y. A. X5 Y% m" DCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal 3 Z2 q5 Y0 v' FForebrain Neural Stem Cells # S& k. L7 \# B! ?' A" }: O5 Y4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage5 j% c+ r2 |; u
Progression, and Maturation, m' Z8 H. s1 T5 M( x! w; B. p) m
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic6 R0 C, i# }/ e1 r+ P( {/ C! l
Neurons from Rodent Brains " b t% r- J5 I, ]6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere$ w; ]2 l6 \2 d# P; s
Technique ! z7 Q* b7 E, n: H1 u9 N7 V5. Section E Germline Stem Cells( ~, y! C# O* h' h0 r+ x
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila 5 H, [( P7 L8 g- V ~- h; t1 G" H2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis8 N% A, t6 ^8 N2 l' G
6. Section F Gut Stem Cells7 u' @4 M4 Y8 |) J7 {9 e
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells 6 V; P& T7 J& k! T# A7. Section G Lung Stem Cells ; r; s# q# H% O. u& V5 Q, S1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells) [/ s" s$ m; U1 _9 b
5. Chapter 3 Cancer Stem Cells) `3 n" ?% Y$ F* `$ f' Y; D6 g
1. Unit 3.1 Colon Cancer Stem Cells2 t' H5 N9 ?; R' y! T8 L
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model F a0 x' o- l3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells - c3 Y( [) E: w+ `/ O# @6. Chapter 4 Manipulation of Potency / u b) {' x' Y \, ^ d3 E7 m! y1. Section A iPS Cells + v. B. l7 \! F+ P1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming / n' `( i' S0 S) }' M2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells6 P& |6 Q( Y- ?% v% Z' e
2. Section B Nuclear Transfer ( }: |) s* N+ E4 o1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency 2 E! \/ s2 y- k0 s3 Q! |7. Chapter 5 Genetic Manipulation of Stem Cells U7 ^( ] O" }* c& Q; H4 G; W4 G
1. Section A Lineage Tracers in Stem Cells6 ?& M* U: l% j: X# q
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma 2 `; H3 k. [6 j1 p! _' e2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing* A9 N( S0 F: C- A7 V
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells9 L7 u) n. s& n; U* ^& C
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium ) ?6 n" e& z7 T& Z! L5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian- g4 [ H0 i1 T7 N9 ~8 n( u
Neocortical Neural Progenitors7 P% N) t% E& h7 G3 z
2. Section B Homologous Recombination in Stem Cells* N) k# g4 V! Y9 F: [4 v3 H8 x
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by ' t W0 a& r5 D4 aHomologous Recombination 3 @7 b3 f& j5 B B' S) [# l( V8. Appendix 1 Useful Information ( V9 T9 O: M# t1 N$ I2 H1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research2 ~3 u! w3 [5 r* p
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells5 Q6 A% {9 J- l/ d$ y5 ?( F( L1 C' u
9. Appendix 2 Laboratory Equipment4 j, L; x3 A4 \, F5 g# _7 M
Standard Laboratory Equipment $ y! X. O5 p: |, G* U10. Appendix Suppliers0 Q4 g0 t: ?- k, h
Selected Suppliers of Reagents and Equipment 8 m5 Z& {2 T% z9 e : Q* D2 X. v* L9 t. D作者: angelyannan 时间: 2011-3-8 20:14
