. t" \' e+ t& C4 Q( MGunsalus及其同事开发了一种基于Java的工具显示基因网络,Oegema等利用这一新技术定量分析了基因与基因之间的相互作用,并将这些信息输入到一个功能基因网络中,从而构建了一个包含818个线虫必需基因的集成功能网络。 ( V( }( E& I% F1 d* l + o& {. M! {, `科学家们称这一网络可用于预测相关人类基因的功能。在接下来的工作中,他们计划利用更广阔的技术生成其他生物体包括脊椎动物的功能基因网络。 ( e0 a2 p2 c! n6 b9 u0 t) z ) n5 N/ V- g& n l* u' \( L3 k6 U3 I2 W
A High-Resolution C. elegans Essential Gene Network Based on Phenotypic Profiling of a Complex Tissue( {+ b9 H! M3 }3 e( r4 ]5 v
6 E, r' z5 w1 _& eHigh-content screening for gene profiling has generally been limited to single cells. Here, we explore an alternative approachprofiling gene function by analyzing effects of gene knockdowns on the architecture of a complex tissue in a multicellular organism. We profile 554 essential C. elegans genes by imaging gonad architecture and scoring 94 phenotypic features. To generate a reference for evaluating methods for network construction, genes were manually partitioned into 102 phenotypic classes, predicting functions for uncharacterized genes across diverse cellular processes. Using this classification as a benchmark, we developed a robust computational method for constructing gene networks from high-content profiles based on a network context-dependent measure that ranks the significance of links between genes. Our analysis reveals that multi-parametric profiling in a complex tissue yields functional maps with a resolution similar to genetic interaction-based profiling in unicellular eukaryotespinpointing subunits of macromolecular complexes and components functioning in common cellular processes. * G0 b( @4 }# f r3 b: U& E3 L X- B6 P
来源:(http://www.ebiotrade.com/) % O4 s$ R2 e& D6 q; l# T , U5 y# I6 {1 X" q5 s. h: k! k 作者: kjeldahl 时间: 2011-4-30 23:47