书名:Human Cell Culture Protocols, 3rd edition (人细胞培养手册,第3版) " m7 ^, _: A; l$ x$ Y3 l o. u出版社:Humana Press 4 a1 _& r) `; O6 N- V
出版年:2012 % Z2 M0 W4 q Q; _ISBN: 1617793663 . w6 s% c0 y: R; T7 M2 Z
页数:449 pages : K% A0 Q; }- q. k |; X
格式:PDF : D; C9 j n6 { ; s e1 l' \, |8 d9 v" |Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research. . ]9 Y9 c! l, y' A/ r ( ?1 [1 `- ^* I/ o% e' e作者: john7812x 时间: 2011-11-13 18:38
kankan作者: lunyuyuanxj 时间: 2011-11-13 18:51
一本书吗作者: soldier 时间: 2011-11-13 19:21
kankan作者: uyunbilig 时间: 2011-11-13 19:23
谢谢分享!作者: woosheng 时间: 2011-11-13 19:33
thanks作者: hnsdlgl 时间: 2011-11-13 19:59
gooooood作者: yxc 时间: 2011-11-13 22:01
看看作者: zorro 时间: 2011-11-13 23:03
1111作者: zzc2211 时间: 2011-11-14 00:35
好东西,谢谢作者: daviddow 时间: 2011-11-14 02:01
谢谢作者: lchanlon 时间: 2011-11-14 02:11
Thank you for your sharing 0 R+ T% {; \4 Y# {$ v, S- Y8 J作者: namulow 时间: 2011-11-14 04:17