第二期网上语音研讨会预告（2010年2月26日）
[i=s] 本帖最后由 细胞海洋 于 2010-2-26 23:11 编辑 [/i][color=Red]干细胞之家网上语音研讨会第二期成功举行（录音已上传) [/color]
下载地址：[url]http://www.stemcell8.cn/thread-17813-1-1.html[/url]
1月15日， Cell Death and Differentiation杂志在线发表了中国科学院上海生命科学研究院/上海交通大学医学院健康科学研究所杨黄恬研究组的最新研究工作“Type 3 inositol 1,4,5-trisphosphate receptor negatively regulates apoptosis during mouse embryonic stem cell differentiation”。
干细胞之家非常高兴的邀请到了杨黄恬研究组的黄吉均研究员，与大家探讨相关研究经验。
主讲题目：三磷酸肌醇受体三在胚胎干细胞分化中的作用
本期内容的详细介绍：[url]http://www.stemcell8.cn/thread-17760-1-1.html[/url]
主讲人介绍：黄吉均
中科院干细胞重点实验室
分子心脏学课题组
中科院上海生科院健康科学研究所
研讨会时间：2月26日晚 8：30开始
参加方法：[url]http://www.stemcell8.cn/thread-12174-1-1.html[/url]
欢迎大家踊跃参与 积极提问
文章
Type 3 inositol 1,4,5-trisphosphate receptor negatively regulates apoptosis during mouse embryonic stem cell differentiation.
Liang J, Wang YJ, Tang Y, Cao N, Wang J, Yang HT.
Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) and Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
Ca(2+) signals generated by inositol 1,4,5-trisphosphate receptors (IP(3)Rs) are crucial for cellular processes such as apoptosis and differentiation. However, the exact roles of IP(3)Rs and their contributions to Ca(2+) signals in pluripotent embryonic stem (ES) cell behaviors remain largely unknown. In this study, we showed that the expression of type 3 IP(3)R (IP(3)R3) was transiently downregulated with a concomitant increase in apoptosis at the early differentiation stage of murine ES cells. Knockdown of IP(3)R3 by small interfering RNA increased apoptosis in differentiating cells but not in undifferentiated ES cells. Moreover, IP(3)R3 overexpression had the opposite effect. Consistently, IP(3)R3 knockdown altered Ca(2+) oscillations in differentiating cells but not in undifferentiated ES cells. The apoptosis in differentiating IP(3)R3-knockdown cells was decreased by chelating intracellular Ca(2+) with BAPTA-AM and increased in control ones. Furthermore, IP(3)R3 knockdown led to a suppression of the expression of mesodermal and mesoendodermal but not ectodermal markers. The differentiation suppressions were further confirmed by the impaired differentiation of mesodermal and some of the endodermal but not ectodermal derivatives. Such defects were partially because of the increased apoptosis in Flk-1(+) cells. These findings provide the first demonstration of the important role of IP(3)R3 in the regulation of apoptosis in early differentiating ES cells and subsequent lineage commitments through modulation of Ca(2+) signals.Cell Death and Differentiation advance online publication, 15 January 2010; doi:10.1038/cdd.2009.209. 期待期待... ... 期待之中....... 很期待啊！自己这方面还不太了解，多多向各位学习！ 多多学习:) 向各位大牛学习了~ 永远支持！ 拿去学习了 thanks！ 来晚了，期待下一期
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