视频教程-人胶质母细胞瘤神经球培养方法
[i=s] 本帖最后由 细胞海洋 于 2012-2-29 00:52 编辑 [/i]视频教程-人胶质母细胞瘤神经球培养方法
Video Article
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells
Using the Neurosphere Assay
Hassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1
1Department of Neurosurgery, University of Florida
2Department of Anatomical Sciences, Shiraz University of Medical Sciences
Correspondence to: Hassan Azari at [email]Hassan.azari@neurosurgery.ufl.edu[/email]
URL: [url]http://www.jove.com/video/3633/[/url]
DOI: 10.3791/3633
Keywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,
Date Published: 10/30/2011
Citation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme
Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011).
Abstract
Stem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in
glioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression,
and recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The
neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of
this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human
GBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
resulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready
for further passaging and expansion.
[color=Red]下载地址：包含PDF和视频
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**** Hidden Message ***** 谢谢楼主，先看看能不能下 卡那看那 [b]回复 [url=forum.php?mod=redirect&goto=findpost&pid=584829&ptid=52919][color=Olive]暮色英雄[/color] 的帖子[/url][/b]
怎么还要注册网盘，太麻烦了
看看咯 kankan 这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：） [quote][size=2][color=#999999]yuye3000 发表于 2012-3-1 23:01[/color] [url=forum.php?mod=redirect&goto=findpost&pid=586518&ptid=52919][img]static/image/common/back.gif[/img][/url][/size]
这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）[/quote]
这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。 谢谢分享 谢谢分享