Powered by gel
Gelled macrophage supernate sticks to the top of an inverted tube (left).STOSSE
optical microscopes had shown that cells engulf food and slither along by turning part of their cytoplasm into a semi-solid gel, and then liquefying it again. Stossel and his colleague John Hartwig (both at Harvard University) wanted to know what controlled this gel–sol transformation.
At the time, the discovery that nonmuscle cells contained actin and myosin was fresh. But what the pushy proteins accomplished was uncertain〞researchers had just discerned that actin helps form the contractile ring that pinches cells in half during division (Schroeder, 1972). Stossel and Hartwig started by nabbing a new molecule they called actin-binding protein〞the very first actin-binding protein〞that spurred actin fibers in vitro to coalesce into a mesh (Hartwig and Stossel, 1975). This mesh later turned out to provide a substrate for myosin-mediated contraction.
Next, Stossel and Hartwig (1976) reproduced this phenomenon with purified proteins and linked the process with what was happening in vivo during phagocytosis. They showed that extracts of macrophages in the midst of phagocytosis solidified into a gel and did so faster than did those from cells that weren't eating. What's more, cytoplasm from cells that had recently swallowed an oil droplet contained more actin-binding protein than did material from resting cells. A mixture of actin, myosin, and actin-binding protein, but not the duo of actin and myosin alone, would also gel.
The idea that actin molecules can't knit into a gel without help from actin-binding protein was controversial, Stossel recalls. In fact, the preceding paper in the same issue argued the opposite view (Pollard, 1976). Stossel says that it took about 15 years to win over most doubters, and during this time the number of participating molecules swelled. For example, Stossel's lab discovered a protein called gelsolin, which unhooks actin filaments (Yin and Stossel, 1979). Gelsolin and the original actin-binding protein, now called filamin A, are two of the hundreds of molecules that help orchestrate cell movements.
Hartwig, J.H., and T.P. Stossel. 1975. J. Biol. Chem. 250:5696–5705.
Pollard, T.D. 1976. J. Cell Biol. 68:579–601.
Schroeder, T.E. 1972. J. Cell Biol. 53:419–434.
Stossel, T.P., and J.H. Hartwig. 1976. J. Cell Biol. 68:602–619.
Yin, H.L., and T.P. Stossel. 1979. Nature. 281:583–596.(Thomas Stossel was lucky he didn't know ) 干细胞疾病模型 这个站不错！！ 我的妈呀，爱死你了 加油站加油 进行溜达一下 回贴赚学识，不错了 应该加分 胚胎干细胞 文笔流畅，修辞得体，深得魏晋诸朝遗风，更将唐风宋骨发扬得入木三分，能在有生之年看见楼主的这个帖子。实在是我三生之幸啊。