不用干细胞和iPS细胞 人皮肤可直接转换成血液

regene

非常棒的工作！我在07年就想要做类似的工作，老板认为风险太大，放弃了；现在很高兴看到此文章，与大家分享～希望此技术早日应用于临床！+ M8 Z0 a  J/ y5 ^
Direct conversion of human fibroblasts to multilineage blood progenitors/ G* F% u* K' E  Y4 ?

4 B  t6 G9 ^/ r7 u* @  s7 @As is the case for embryo-derived stem cells, application of reprogrammed human induced pluripotent stem cells is limited by our understanding of lineage specification. Here we demonstrate the ability to generate progenitors and mature cells of the haematopoietic fate directly from human dermal fibroblasts without establishing pluripotency. Ectopic expression of OCT4 (also called POU5F1)-activated haematopoietic transcription factors, together with specific cytokine treatment, allowed generation of cells expressing the pan-leukocyte marker CD45. These unique fibroblast-derived cells gave rise to granulocytic, monocytic, megakaryocytic and erythroid lineages, and demonstrated in vivo engraftment capacity. We note that adult haematopoietic programs are activated, consistent with bypassing the pluripotent state to generate blood fate: this is distinct from haematopoiesis involving pluripotent stem cells, where embryonic programs are activated. These findings demonstrate restoration of multipotency from human fibroblasts, and suggest an alternative approach to cellular reprogramming for autologous cell-replacement therapies that avoids complications associated with the use of human pluripotent stem cells.2 q6 h5 F& I: n2 \" e
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