那篇不用转录因子的 重编程终于出来文献了（附原文）

上海过客

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Mol Ther. 2010 Oct 26. [Epub ahead of print]
. H% F$ s+ S. p% ^+ z7 a% U6 ZLentivirus-mediated Reprogramming of Somatic Cells in the Absence of Transgenic Transcription Factors.
8 a4 X$ z  E8 QKane NM, Nowrouzi A, Mukherjee S, Blundell MP, Greig JA, Lee WK, Houslay MD, Milligan G, Mountford JC, von Kalle C, Schmidt M, Thrasher AJ, Baker AH.
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British Heart Foundation Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
% K$ D, e' x5 ^Abstract1 a& X  `( {1 \5 ^2 L+ t
Retroviral vectors remain the most efficient and widely applied system for induction of pluripotency. However, mutagenic effects have been documented in both laboratory and clinical gene therapy studies, principally as a result of dysregulated host gene expression in the proximity of defined integration sites. Here, we report that cells with characteristics of pluripotent stem cells can be produced from normal human fibroblasts in the absence of reprogramming transcription factors (TFs) during lentiviral (LV) vector-mediated gene transfer. This occurred via induced alterations in host gene and microRNA (miRNA) expression and detrimental changes in karyotype. These findings demonstrate that vector-induced genotoxicity may alone play a role in somatic cell reprogramming derivation and urges caution when using integrating vectors in this setting. Clearer understanding of this process may additionally reveal novel insights into reprogramming pathways.- `/ {; d7 X  P0 x3 o1 M5 L

; n, O( o9 k9 h! l# Y8楼原文 感谢snokey提供

上海过客

前段时间，回答论坛问题时，就用了这一信息，不过没有证据，终于有了，谢谢
  Y( G" P. a1 Y0 U6 L. j2 Eexin11 发表于 2010-10-30 08:23

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. K) j2 R/ C8 e3 {- \# S( i& q    我也是 ，一直关注这个信息 。
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    如果有更多人重复这个实验的话 ，可就有意思了 。' l' ^8 O1 F( q* ^( c* c
     这个会掀起  新一轮的 iPS 研究的 热潮 。不，应该是 对细胞脱分化研究的热潮 。