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是不是这篇啊？？有人发过了~~~
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诱导的多能干细胞（iPSCs）为艾滋病的治疗提供了希望, @& W. L& ]# z# `- T4 w
http://www.stemcell8.cn/thread-36638-1-1.html
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. V0 Z0 a) L+ fMol Ther. 2011 Mar;19(3):584-93. Epub 2010 Nov 30.+ [+ z9 p2 s0 c, R
! a/ t: I% c$ u% l; f M( h2 gGeneration of HIV-1 Resistant and Functional Macrophages From Hematopoietic Stem Cell-derived Induced Pluripotent Stem Cells.
3 p& y. E. h$ S6 N4 X" K; jKambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS.
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; c, k# }& Z4 g- @Stem Cell Program, Department of Internal Medicine, University of California, Davis, Sacramento, California, USA.% o" T1 p) E% T7 ^
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Abstract9 n4 t3 {" P; [$ t' U j
Induced pluripotent stem cells (iPSCs) have radically advanced the field of regenerative medicine by making possible the production of patient-specific pluripotent stem cells from adult individuals. By developing iPSCs to treat HIV, there is the potential for generating a continuous supply of therapeutic cells for transplantation into HIV-infected patients. In this study, we have used human hematopoietic stem cells (HSCs) to generate anti-HIV gene expressing iPSCs for HIV gene therapy. HSCs were dedifferentiated into continuously growing iPSC lines with four reprogramming factors and a combination anti-HIV lentiviral vector containing a CCR5 short hairpin RNA (shRNA) and a human/rhesus chimeric TRIM5α gene. Upon directed differentiation of the anti-HIV iPSCs toward the hematopoietic lineage, a robust quantity of colony-forming CD133(+) HSCs were obtained. These cells were further differentiated into functional end-stage macrophages which displayed a normal phenotypic profile. Upon viral challenge, the anti-HIV iPSC-derived macrophages exhibited strong protection from HIV-1 infection. Here, we demonstrate the ability of iPSCs to develop into HIV-1 resistant immune cells and highlight the potential use of iPSCs for HIV gene and cellular therapies.& c- z @( F5 }; B$ C' U `8 }' i& I
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发表于 2011-3-24 16:50
