this paper highlights the chromosomal methylomic differences of iPSC from different adult somatic cell origins versus those from fetal or nuclear transfer in the differentiation to blood cells. there are still a lot of questions regarding what may turn different somatic cells to authentic ground state or be identical to those of totipotent stem cells - chemically, it has to be validated with methylome of key differention genes while it must be qualified by successful production of pedigrees of reproductive animals.
According to the authors, other potential differences before ES and iPS lie in the chromatin modifcations, especially concerning bivalent chromatin domains around developmental genes, and also methylation status at some pioneer factor binding sites in enhancer (but not promter) regions of some tissue specific genes.
发表于 2010-7-31 20:17
发表于 2010-8-1 15:52
