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本帖最后由 hyde 于 2011-1-21 21:53 编辑 - `+ |! R* g5 T1 c! s6 ]5 j
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Pig Epiblast Stem Cells Depend on Activin/Nodal Signaling for Pluripotency and Self-Renewal% O# ]' A+ [4 R/ [
pEpiSC通过Activin/Nodal Signaling信号途径来维持多能性和自我更新能力
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Ramiro Alberio , Nicola Croxall , and Cinzia Allegrucci
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7 {& K: |- {& }" q6 W+ ~1.说明：原文来源自http://www.liebertonline.com/doi/abs/10.1089/scd.2010.0012
6 x- ~8 ]; Y3 X5 x3 T+ u" G* g由干细胞之家新闻小组成员Hyde翻译（转帖请注明）
$ r! L+ v8 G0 u9 Y0 H+ S2.翻译内容
; [/ a% [" B7 l! s; ImEpiSC和hESC通过Activin/Nodal signaling信号途径来维持细胞的多能性和自我更新能力。我们想要通过研究获知该信号系统在pEpiSC中的作用。我们取10.5-12天的猪胚来建立pEpiSC细胞系。从获得的胚胎中分离出epiblast。其中使用小鼠灭火成纤维细胞（MF）做为饲养细胞，并在培养基中补充bFGF。获得的pEpiSC表达多能性核心标准因子，如 OCT4 (或POU5F1 )、NANOG 、SOX2 和 NODAL。但是监测不到 REX1 和AP（碱性磷酸酶）活性。为了研究 leukemia inhibitory factor (LIF)/JAK/STAT3 pathway信号途径对pEpiSC多能性和自我更新能力的影响，我们在培养基中分别加入特异性的抑制剂JAK I inhibitor 420099和LIF的抗体。pEpiSC的多能性在多次传代培养（>3）后都未出现明显的变化。相反，在培养基中加入针对 Activin/Nodal pathway的抑制剂Alk-5 inhibitor B431542，pEpiSC出现向神经细胞分化的现象。 pEpiSC 是具有多能性的，它们可以自发分化为内胚层、外胚层、中胚层组织。在诱导条件下如BMP-4，还可以分化为滋养层细胞和生殖系细胞前体。我们的研究发现猪的 epiblasts 能够表达多能性的核心标准因子，同时 pEpiSC 的自我更新能力与Activin/Nodal signaling信号途径相关。这项研究也进一步证明了通过Activin/Nodal signal信号途径来保持多能性在不同的哺乳生物之间具有一定的保守性。, f3 ~) O2 M. p6 r9 @+ D
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Activin/Nodal signaling is required for maintaining pluripotency and self-renewal of mouse epiblast stem cells0 k; s, \' @6 u: k/ e$ Y3 Q
and human embryonic stem cells (hESC). In this study, we investigated whether this signaling mechanism is
4 \' d' T3 `/ `also operative in cultured epiblasts derived from Days 10.5–12 pig embryos. Pig epiblast stem cell lines (pEpiSC)" R% X) }$ \5 o
were established on mouse feeder layers and medium supplemented with basic fibroblast growth factor (bFGF).
6 E" L7 T6 ^% k8 z2 B4 apEpiSC express the core pluripotency factors OCT4 (or POU5F1 ), NANOG , SOX2 , and NODAL , but they do not% R5 W: C7 K3 B. d4 E/ V
express REX1 or alkaline phosphatase activity. Blocking leukemia inhibitory factor (LIF)/JAK/STAT3 pathway
1 d: U; U! _5 b5 T$ z! ^by adding the specific JAK I inhibitor 420099 and an anti-LIF antibody over 3 passages did not affect pluripotency
$ [/ R& x( s4 a% ?+ d1 Qof pEpiSC. In contrast, cells grown with the Alk-5 inhibitor SB431542, which blocks Activin/Nodal
, G% R9 [+ B# U" J {% m5 Dpathway, differentiated readily toward the neural lineage. pEpiSC are pluripotent, as established by their differentiation) t Q3 ~ ?% E
potential to ectoderm, mesoderm, and endoderm. These cells can be induced to differentiate toward
1 U* ?- W* G; j6 Rtrophectoderm and to germ cell precursors in response to bone morphogenetic protein 4 (BMP-4). In conclusion,) F6 I. p6 [" E& u- r! R8 q
our study demonstrates that pig epiblasts express the core pluripotency genes and that the capacity for maintaining3 Z7 J4 l! w! _$ R( z" n
self-renewal in pEpiSC depends on Activin/Nodal signaling. This study provides further evidence that
1 O9 E: {7 t- |( Imaintenance of pluripotency via Activin/Nodal signal is conserved in mammals.0 F- e9 z$ m R# S3 X: C
4.上传原文附件
+ [1 Y% [. K; A3 l5.个人翻译，理解不准确的语句还望大家积极指出。
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发表于 2011-1-21 21:15
发表于 2011-1-21 21:34
