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本帖最后由 hyde 于 2011-1-21 21:53 编辑
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Pig Epiblast Stem Cells Depend on Activin/Nodal Signaling for Pluripotency and Self-Renewal
' H3 G* f* ~# {) `9 t" _/ S5 `# `pEpiSC通过Activin/Nodal Signaling信号途径来维持多能性和自我更新能力
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Ramiro Alberio , Nicola Croxall , and Cinzia Allegrucci - H" f" a0 W+ ]* M" U5 _
7 U0 H4 _; Y+ M8 C1.说明：原文来源自http://www.liebertonline.com/doi/abs/10.1089/scd.2010.00120 T: O9 b- B8 n! K
由干细胞之家新闻小组成员Hyde翻译（转帖请注明）
- G0 F& A- w2 N/ h' \1 |2.翻译内容4 f2 j0 N9 }& s- c6 c8 ]/ y
mEpiSC和hESC通过Activin/Nodal signaling信号途径来维持细胞的多能性和自我更新能力。我们想要通过研究获知该信号系统在pEpiSC中的作用。我们取10.5-12天的猪胚来建立pEpiSC细胞系。从获得的胚胎中分离出epiblast。其中使用小鼠灭火成纤维细胞（MF）做为饲养细胞，并在培养基中补充bFGF。获得的pEpiSC表达多能性核心标准因子，如 OCT4 (或POU5F1 )、NANOG 、SOX2 和 NODAL。但是监测不到 REX1 和AP（碱性磷酸酶）活性。为了研究 leukemia inhibitory factor (LIF)/JAK/STAT3 pathway信号途径对pEpiSC多能性和自我更新能力的影响，我们在培养基中分别加入特异性的抑制剂JAK I inhibitor 420099和LIF的抗体。pEpiSC的多能性在多次传代培养（>3）后都未出现明显的变化。相反，在培养基中加入针对 Activin/Nodal pathway的抑制剂Alk-5 inhibitor B431542，pEpiSC出现向神经细胞分化的现象。 pEpiSC 是具有多能性的，它们可以自发分化为内胚层、外胚层、中胚层组织。在诱导条件下如BMP-4，还可以分化为滋养层细胞和生殖系细胞前体。我们的研究发现猪的 epiblasts 能够表达多能性的核心标准因子，同时 pEpiSC 的自我更新能力与Activin/Nodal signaling信号途径相关。这项研究也进一步证明了通过Activin/Nodal signal信号途径来保持多能性在不同的哺乳生物之间具有一定的保守性。
4 q, p+ `3 z! @4 T, b u% s* y3.原文
' F8 x9 Z4 S. G- yActivin/Nodal signaling is required for maintaining pluripotency and self-renewal of mouse epiblast stem cells
/ l: {6 K$ a( jand human embryonic stem cells (hESC). In this study, we investigated whether this signaling mechanism is1 J* C3 K3 H; l6 b7 U
also operative in cultured epiblasts derived from Days 10.5–12 pig embryos. Pig epiblast stem cell lines (pEpiSC)$ b+ A H* P4 @+ E9 k0 ?( H
were established on mouse feeder layers and medium supplemented with basic fibroblast growth factor (bFGF).4 G m" T0 v" }% [" m& I) l& V
pEpiSC express the core pluripotency factors OCT4 (or POU5F1 ), NANOG , SOX2 , and NODAL , but they do not; n& e) M! Y: F: T; u9 z. ^# c
express REX1 or alkaline phosphatase activity. Blocking leukemia inhibitory factor (LIF)/JAK/STAT3 pathway
1 p9 Z) K* O4 S1 h( i* u" Aby adding the specific JAK I inhibitor 420099 and an anti-LIF antibody over 3 passages did not affect pluripotency' N9 X, a8 _* w$ a8 L! K
of pEpiSC. In contrast, cells grown with the Alk-5 inhibitor SB431542, which blocks Activin/Nodal) m0 a6 j" D4 I, |
pathway, differentiated readily toward the neural lineage. pEpiSC are pluripotent, as established by their differentiation% V7 H1 Z6 p1 v T3 \
potential to ectoderm, mesoderm, and endoderm. These cells can be induced to differentiate toward
$ {6 `; P& `" l" E7 P7 m( e' _- Dtrophectoderm and to germ cell precursors in response to bone morphogenetic protein 4 (BMP-4). In conclusion,
0 N3 S: W# ], O$ ^2 a$ H# Uour study demonstrates that pig epiblasts express the core pluripotency genes and that the capacity for maintaining
1 J- }8 r0 f. |self-renewal in pEpiSC depends on Activin/Nodal signaling. This study provides further evidence that
) m: c8 ~2 d( Xmaintenance of pluripotency via Activin/Nodal signal is conserved in mammals.$ `$ C. m$ \5 Z/ t$ ?$ h
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5.个人翻译，理解不准确的语句还望大家积极指出。
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发表于 2011-1-21 21:15
发表于 2011-1-21 21:34
