旧菜翻炒——关于细胞冻存

hub12333

我冻的细胞一般都是4-5小时就从-80到液氮，复苏后细胞也可以，没得太大问题

shashou_com

这个没试过，下次你试的时候记得给大家说下结果。

houbara

1. the time in -80c, depends on the cryo-vial and temperature decreasing rate. if the temperature decreasing at -1C per minute, eg, Bicell. it would be more than enough to keep cells in -80 for 4hours. To cryopreserve cells for a short time,1-3 mon, you can just leave it in -80c
0 H0 N# k3 I' |% g3 |6 d+ I, y# R2. For longterm preservation, it is better to transfer your cells into LN2- }# T% K& p% H1 w
3. Overnight in -80, it is just for the work convinience. 5 |1 d( Y- H( e- f: P+ ?' N

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! ^/ x8 H# z" L. v" p: @" B! e3 |过夜是为了好安排工作，-80 放4小时就可以