PDF电子书：Current Protocols in Stem Cell Biology 2007

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FOREWORD. z7 Q# e; v( y" Z: K9 [8 j' E5 k
Stem cell biology is emerging as a field in biology with tremendous therapeutic potential.
6 {# x+ T0 C+ V. ]Making this potential a reality requires an international effort. The recognition) d9 x* M% b! ~/ Z/ l. o2 b
that such a promising yet multifaceted discipline needs fostering led to the establishment
8 y8 L, S4 S/ q1 T* r1 \' [  h8 oof the International Society for Stem Cell Research (ISSCR). Central to the efforts of the
5 s; \, }( y: ?ISSCR is the development of tools to ensure the success of stem cell researchers. What
8 w4 y/ Y& H) H: u: c( q0 E1 Ubetter way to do this than to collaborate with Current Protocols to develop this valuable- b  ~$ ]( s' w: N
compendium of protocols in stem cell biology?
3 k* l9 X0 y  A% }0 B4 p! @) W9 ^Stem cell researchers have developed a number of breakthrough techniques, from the+ e; I' g( K- r: F; u2 O9 N
derivation and manipulation of pluripotent embryonic stem cells to purification and# s) e+ G/ `# ^4 s* c% y# T
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories( t3 h: ]& d9 b. V2 I  C
have become leaders in the field as a result of developing such techniques. The more
- S; E/ ?( @. W4 uefficient scientists are at implementing new and powerful methodologies in their own, Q$ D" o+ ]' f# z5 j; I
laboratories, the faster stem cell biology will advance our understanding of normal+ q+ ~- ~+ [9 j0 {# y" Z
development and lead to the development of therapies. Thus, the availability of quality
. Y. [* }' E6 o- l! |" `protocols will have amajor impact on the success of the entire field. Current Protocols has- F: v( P0 N+ v8 p3 {8 c9 ?
long been the premier volume for proven in-depth protocols regarding many aspects of
6 }  Q# M4 q; [1 c, Bbiology, and this volume on stem cell biology will prove a valuable addition to researchers0 G' f$ x, t0 ~" j
worldwide.
! {" F4 |6 G9 F3 tExperiments in stem cell biology must be interpreted with great caution as well as
9 D/ i4 s8 o, @  O0 lopenness to alternative explanations. For example, the recently discovered phenomenon; F. W" S4 c* w0 g/ i  `
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
1 N$ k) v* X( l% Z4 e! Y# L( O# |1 ]; Itissues were initially misinterpreted as evidence for stem cell trans-differentiation.$ u  E& j* R( w' s! w0 p- o
It is very important that this compendium of protocols highlight potential pitfalls as well3 h' `# R0 P! x+ ]
as maintain the opportunity for clarification and correction when the need arises. The
9 S9 U$ e0 t/ n, Zfact that these protocols will be provided online will help ensure that researchers always
  a" N+ B4 U7 O4 z4 E2 \1 rhave the latest, most up-to-date protocols available to them.
9 L! G8 t* T$ z/ M0 EThe stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine
/ \) L- f! r7 y9 n* w% @6 d4 {7 F$ t, Tpush to share information and interact so that the field can move forward quickly. There  M; u/ k% u0 _. Z2 y: Y  ]/ H& P* \
is a drive to develop not only excellent basic research skills but to bring the findings6 @2 Q  f  h( E; v
to clinical use so that patients can benefit. As a Hematology Attending Physician at7 I& o% [: U/ F" H4 J5 \5 k
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia
$ b5 L" ?  \8 T! h. f$ U' Hand am drawn by the need to translate our research findings into therapies to help treat a0 k4 j& \# n  f, l" K, z; ~) _
number of diseases. The promise is great, but we need to deliver, and I believe Current7 l3 O2 H, [  D' _( M
Protocols in Stem Cell Biology will help tremendously.9 I9 v/ m$ J  \
Leonard I. Zon
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