标题: DNA methylation dynamics in human induced pluripotent stem cells [打印本页] 作者: Tlexander 时间: 2016-4-19 12:30 标题: DNA methylation dynamics in human induced pluripotent stem cells
DNA methylation dynamics in human induced pluripotent stem cells + V g8 U+ V" A7 M6 [" I7 j8 {+ ?5 Z V& Q$ Q9 s a: c6 n
[attach]77105[/attach] 5 E' y% |8 u& J# l* `/ l* _& C; G# ?; D: ~% T
/ e+ N- }" d- s- w d
Abstract Indeed human induced pluripotent stem cells) g! I8 w5 S" H/ M- q0 I/ [
(hiPSCs) are considered to be powerful tools in regenerative 4 ?* J, H. ]& o. n( t% Kmedicine. To enable the use of hiPSCs in the field of 2 u; B3 U# E+ o% d6 Vregenerative medicine, it is necessary to understand the $ e$ z7 j+ a3 wmechanisms of reprogramming during the transformation ; V, ^# e2 s) t# s3 z xof somatic cells into hiPSCs. Genome-wide epigenetic$ n5 i9 V% O- B% F) o3 D) g) M
modification constitutes a critical event in the generation of 5 T2 j2 R2 x# h; z* v4 Z9 G8 ~iPSCs. In other words, to analyze epigenetic changes in: |6 X+ z/ M% t! d& U3 |% S
iPSCs means to elucidate reprogramming processes. We8 j$ g0 {- X, ~
have established a large number of hiPSCs derived from% _3 Y w) B, d; | H
various human tissues and have obtained their DNA 2 F8 g T2 B6 J/ Nmethylation profiles. Comparison analyses indicated that 0 P# u9 M( W; c! S$ {8 q1 _the epigenetic patterns of various hiPSCs, irrespective of# K) \. Y0 @5 A
their source tissue, were very similar to one another and9 o2 F- c$ W5 x, r" W7 J! ?
were similar to those of human embryonic stem cells3 m# ]% n+ @3 P& X5 z3 |" M
(hESCs). However, the profiles of hiPSCs and hESCs 0 a/ o. c' y3 C1 o+ W0 p/ p* qexhibited epigenetic differences, which were caused by 5 s( R. r' T& a2 I, w5 @4 Srandom aberrant hypermethylation at early passages. - ]/ M* r; H1 {/ EInterestingly, continuous passaging of the hiPSCs diminished# p) s& D2 m" u' a5 n
the differences between DNA methylation profiles of3 H6 n4 p- E2 T! v5 N; N
hiPSCs and hESCs. The number of aberrant DNA methylation0 w5 \ L6 F4 m9 C# m9 Z+ d9 j, `
regions may thus represent a useful epigenetic index