本帖最后由 细胞海洋 于 2010-1-17 21:00 编辑 . w( X9 m# N9 a. [# u" B l" [- J
" \- g N" E5 U) B8 }' j# d: iPreface! e9 { O) Z& R4 A* t
For several decades, Arabidopsis thaliana has been the organism of choice in the ! E2 H$ y2 v( W! Y# t3 }, [laboratories of many plant geneticists, physiologists, developmental biologists, and 5 C; C) x8 Y; Gbiochemists around the world. During this time, a huge amount of knowledge has been$ L8 s2 F& r" _) c' |* K3 u" ~
acquired on the biology of this plant species, which has resulted in the development of ( m; R0 x3 g* @& c7 tmolecular tools that account for much more efficient research. The significance that # ^' u4 t3 t& ]; q# Y( q5 Z: \Arabidopsis would attain in biological research may have been difficult to foresee in7 B* L- \: D, B4 [5 e" k
the 1980s, when its use in the laboratory started. In the meantime, it has become the: ]( D4 z/ }: O/ e; l% ^5 B! j2 }
model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse 8 r' L; O6 y8 H2 B9 }have for animal systems. Today, it is difficult to envision research at the cutting edge of 4 X/ R- [8 z$ u4 n2 G' n5 c; w# [plant biology without the use of Arabidopsis.1 Q. D0 q* C) z% l# [
Since the first edition of Arabidopsis Protocols appeared, new developments have / g4 e6 x9 F9 j( r6 N! Afostered an impressive advance in plant biology that prompted us to prepare Arabidopsis 6 z) q9 J# G3 I4 E( V0 c. yProtocols, Second Edition. Completion of the Arabidopsis genome sequence offered for ) R% [4 |* J, a& K( @+ Nthe first time the opportunity to have in hand all of the genetic information required for& M& @, I/ k/ M' k. q& i* Z
studying plant function. In addition, the development of whole systems approaches that 7 d' R; |: U# r' h8 jallow global analysis of gene expression and protein and metabolite dynamics has - h8 U( e C* a1 m* hencouraged scientists to explore new scenarios that are extending the limits of our' U: L; z9 f: w% ]4 ^$ X4 d/ ^
knowledge. These advances will eventually lead to an understanding of how this complex, ) Z( L: [+ I, k) s9 [0 A: b% T0 dmulticellular organism works, how it copes with the particularities of a sessile life W; N8 d* X2 U$ `7 tstyle, and how these strategies compare with those developed in other organisms.! v W7 z5 F+ S/ I( P
While conceiving this second edition of Arabidopsis Protocols, our aim remained as it# ^# \2 w* [7 o0 z( \" \' @
was in the first: the book should provide both experienced researchers and beginners in! y Y6 p" e# i0 V# J. J, F
the field of plant biology with a comprehensive set of up-to-date protocols covering the , E5 s% k) I$ Y0 _. }, H: D5 I3 l6 Ymany methods developed for work with this species. Readers should have little problem$ V$ ?& a( W" `
understanding the general design or the specific details of the experimental protocols.* l: Q- o8 |% }6 R" R$ u
Nevertheless, we recommend reading the protocols in advance, before bench work is" u& ^# ~$ x7 t) `2 ]
started. Although a few chapters in this edition have been maintained and updated, most# l: b1 i, p/ z
are new and describe technologies that have only very recently been developed in ( B4 W/ `# q! p- r$ g: ~( m2 p2 iArabidopsis. We hope that Arabidopsis Protocols, Second Edition will continue as a * r% N/ L' V- Y bstandard reference for laboratories working with Arabidopsis and other plant species./ c5 E2 E+ P4 B" p/ m* g
Julio Salinas" B2 G' i/ q4 t; M8 E4 L
Jose J. Sanchez-Serrano# L3 T* S* v6 b6 J7 ]$ X* H V
因为有些大，我把它分成了2部分。下载之后PDF软件合并一下就可以成整本了哈。作者: 细胞海洋 时间: 2010-1-14 23:57
5 O7 H6 `$ h4 J# z5 h- L' s你现在的级别是新手上路，需要到达注册会员级别才可以下载电子书专区的附件 ! ^4 e c b8 s- e( A # q9 }- R. x: T8 Z2 G l; s. M新手上路升级注册会员级别需要威望达到502 p" k( f B$ r6 q- r9 Q
，那位哥们能下载了给我发过来，谢谢啦！wheatman.wsu@gmail.com