本帖最后由 细胞海洋 于 2010-6-19 13:37 编辑 1 d3 O/ w6 U% X3 l& ?; R: j0 v0 H! p1 \& n- H$ O* n
FOREWORD) t. i' w& S: z' W6 B
Stem cell biology is emerging as a field in biology with tremendous therapeutic potential.- Q3 r! q A7 A/ y; r1 i, i
Making this potential a reality requires an international effort. The recognition 1 f+ [% @% y5 X4 B7 Gthat such a promising yet multifaceted discipline needs fostering led to the establishment* I1 X9 x0 |) a$ W F- j3 @/ c
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the3 B; X: S: V3 T3 ~
ISSCR is the development of tools to ensure the success of stem cell researchers. What& {* }/ ?6 S, } h+ ^0 u) f
better way to do this than to collaborate with Current Protocols to develop this valuable6 ~3 q h8 o7 k# y |2 T0 W
compendium of protocols in stem cell biology? & f) j5 X' n+ ]4 |! P; c" [! BStem cell researchers have developed a number of breakthrough techniques, from the+ T! }! v0 w7 M9 b, z* j
derivation and manipulation of pluripotent embryonic stem cells to purification and & _7 J( q( Z) D/ _, I1 ^transplantation of tissue-restricted stem cells from adult organs. A number of laboratories2 D& h6 d3 I" B7 Q& @4 k3 N9 Q
have become leaders in the field as a result of developing such techniques. The more1 C8 a1 x* e2 [+ b& u
efficient scientists are at implementing new and powerful methodologies in their own/ |+ J/ T" z) C) f8 x
laboratories, the faster stem cell biology will advance our understanding of normal 0 E4 W: a5 W; B( l2 a9 ]. Ydevelopment and lead to the development of therapies. Thus, the availability of quality' a8 u" E' g7 |# d% ]) Q$ w8 M+ \
protocols will have amajor impact on the success of the entire field. Current Protocols has 5 W( U( F, \; ]long been the premier volume for proven in-depth protocols regarding many aspects of % B" J4 c& ^7 N! O5 [- K, D: _$ \. Lbiology, and this volume on stem cell biology will prove a valuable addition to researchers$ o8 G& w+ v; F
worldwide.' b$ q9 h5 ^& g5 e- J' F
Experiments in stem cell biology must be interpreted with great caution as well as: ?- o4 r7 r$ |( C E3 f( t
openness to alternative explanations. For example, the recently discovered phenomenon6 M/ }# T7 n9 W1 }: X
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral% {8 {* L" I1 e8 Z/ h
tissues were initially misinterpreted as evidence for stem cell trans-differentiation.$ X" L" o& i$ k, b3 T
It is very important that this compendium of protocols highlight potential pitfalls as well* H( N4 k9 Z! q7 a% E i+ ]
as maintain the opportunity for clarification and correction when the need arises. The 0 [2 v2 _+ \" v8 ], t" y" [fact that these protocols will be provided online will help ensure that researchers always # f! h l6 z, d9 R7 ?have the latest, most up-to-date protocols available to them. 6 Q) d3 \: }! L: W& f8 tThe stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine) V- g3 ^) _( D- E* P; j6 Q
push to share information and interact so that the field can move forward quickly. There+ ?! Z' z" J3 q+ z2 V# I; M) i- Q( G8 k
is a drive to develop not only excellent basic research skills but to bring the findings- W2 a0 X/ c5 L" ~% V9 I
to clinical use so that patients can benefit. As a Hematology Attending Physician at ; s+ n* {3 \1 e% g- t& {: tChildren’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia $ _' h1 F$ k" {+ ]+ Y6 sand am drawn by the need to translate our research findings into therapies to help treat a , Y& z' t+ Y. h) l0 _3 Lnumber of diseases. The promise is great, but we need to deliver, and I believe Current3 ^7 w7 ]0 Y7 Z6 W( n" D
Protocols in Stem Cell Biology will help tremendously. ) m P; D+ \: T+ _( Q1 |( R3 q* P1 }( }! WLeonard I. Zon p, p) A$ T, F3 B2 a
Current Protocols in Stem Cell Biology ; k5 R/ m- d( ~Published online June 2007 in Wiley Interscience (www.interscience.wiley.com).0 k- r# P+ ], n
DOI: 10.1002/9780470151808.scfores17 Z6 t- M* n8 B9 |5 n- p7 n
Copyright C 2007 John! O$ W" g0 L3 v; o* T! x
8 K9 T- A2 H2 P g w附件为pdf全文，感谢原创提供者 ，大家回复之后就可以下载了1 d' z6 X5 K( q' J) b2 K, D 作者: xiaodongqin 时间: 2009-3-16 08:51
太好了作者: Jonathan 时间: 2009-3-19 17:38
。。。。。。。。。。。。。。作者: zpzp0312 时间: 2009-3-20 15:01
谢谢楼主！作者: zisu_yu 时间: 2009-3-23 10:29
好东东哦。谢谢啦哈作者: 王乾 时间: 2009-3-23 20:57
感谢你的大力支持！作者: horst 时间: 2009-3-24 08:03
真是好资料，很有帮助，谢谢了作者: dragon513 时间: 2009-4-9 11:48
谢谢分享！作者: hualin840518 时间: 2009-6-10 13:16
作者: bobo10679888 时间: 2009-7-2 22:23
作者: 大漠 时间: 2009-7-28 09:23
太需要啦，谢谢！作者: andey 时间: 2009-7-29 12:03
支持 谢谢分享作者: cdcliwei 时间: 2009-7-29 20:38
1#guokeyi5 h/ k0 m4 W+ U( `( Z$ t& D
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I am waitiing!作者: janechen 时间: 2009-7-30 10:01
谢谢分享！