Stem Cells International$ ~/ A) P* J3 s2 X2 Y! \2 E
Volume 2011, Article ID 504723, 14 pages8 D! N9 h6 Q: Z
doi:10.4061/2011/504723& Y* {1 q4 J# z: U) i( W8 C
“Humanized” Stem Cell Culture Techniques:& x: J+ o" {* Z% ]! H8 d. s
The Animal Serum Controversy ( p/ }( ^( T/ J, WCellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to * H8 {& _4 Z) N& Y/ qregenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, ) U6 R) M) z4 O# r9 X: n8 a. @adipose tissue, and so forth, bring forth the need for their in vitro culture/expansion. However, the extensive usage of supplements ( ^8 e( M. \* ccontaining xenogeneic components in the expansion-media might pose a risk to the post-transplantation safety of patients.7 f. i1 e2 n& ~& H' |& J6 ^ [2 t
This warrants the necessity to identify and develop chemically defined or “humanized” supplements which would make in vitro7 C, ]. A7 g8 O: Z
cultured/processed cells relatively safer for transplantation in regenerative medicine. In this paper, we outline the various caveats 1 ]3 J- B2 Q3 ~& J4 g3 c0 Aassociated with conventionally used supplements of xenogenic origin and also portray the possible alternatives/additives which / D1 l3 v! x s( Wcould one day herald the dawn of a new era in the translation of in vitro cultured cells to therapeutic interventions.作者: hygene 时间: 2011-4-11 21:35
