4 I4 r/ l3 L/ G1 m) L, OStrontium Enhances Osteogenic Differentiation of Mesenchymal Stem Cells and In Vivo Bone Formation by Activating Wnt/Catenin Signaling ~: s. }" x3 ^; f . r& [* _ C' _/ M" z* i) ?FAN YANG, DAZHI YANG, JIE TU, QIXIN ZHENG, LINTAO CAI, LIPING WANGa a5 H) \$ G- m( v+ c
3 g B! J" o* O% @Research Center for Neural Engineering, Shenzhen Key Laboratory for Neuropsychiatric Modulation,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; 4 N5 D2 K2 j4 k* [) k' U9 L4 nDepartment of Orthopaedics, Union Hospital, Tongji Medical College, HuaZhong University of Science and Technology, Wuhan, China; 0 b* N7 c8 ]8 e) cShenzhen Key Laboratory for Cancer Nanotechnology, Shenzhen Institutes of Advanced Technology, : w1 m9 a# e7 n3 ~' R9 ?3 sChinese Academy of Sciences, Shenzhen, China3 J$ M# w$ n z1 m
3 }5 R4 p7 Z. Z$ k: a Strontium ranelate is a newly approved drug that can reduce the risk of vertebral fracture, which is attributed to its dual function in increasing the bone formation and decreasing the bone resorption. Strontium-containing hydroxyapatite was also demonstrated to stimulate the 2 t! |& Y3 f1 r- k0 R8 Z! Bosteoblast activity and inhibit the osteoclast activity.However, the molecular mechanisms of strontium underly- ing such beneficial effects were still not fully understood.5 z( \% l2 A% i* g
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In this study, we investigated the effects of strontium on the osteogenic differentiation of human mesenchymal stem cells (MSCs) and its related mechanism; its osteogenic potential was also evaluated using a calvarial defect model in rats. We found that strontium could enhance' l- p+ B5 B& F; P
the osteogenic differentiation of the MSCs, with upregulated extracellular matrix (ECM) gene expression and activated Wnt/b-catenin pathway. After transplanting the collagen-strontium-substituted hydroxyapatite scaffold into the bone defect region, histology and computed to mography scanning revealed that in vivo bone formation was significantly enhanced; the quantity of mature and remodeled bone substantially increased and ECM accumulated. Interestingly, strontium induced an increase of bcatenin expression in newly formed bone area. In this4 e6 \1 F) _- t
study, we showed for the first time that strontium could stimulate the b-catenin expression in vitro and in vivo, which might contribute to the enhanced osteogenic differentiation of MSCs and in vivo bone formation. STEM CELLS 2011;29:981–991" v2 Y# v/ L( K7 P! a* v, ~1 C& d4 a X
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[attach]27382[/attach] 4 {8 @; X* [3 S% y2 y( s8 i! U! E- v0 W 作者: lifangamanda 时间: 2011-5-24 21:08