标题: ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture [打印本页] 作者: dahui 时间: 2011-9-1 15:17 标题: ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture
本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑 ' ^& h! |- O9 `7 Q
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Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition! E$ s9 k- x2 ?' T
, [9 V# G, t& HIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories./ ^& U5 v) ~1 M* u
* I( f! V- r9 RChapters Title0 E" k# X* u' D% i
1 Introduction( c8 q9 T' _( E/ n, n# W
2 Design and Equipment for the Cell Culture Laboratory2 H+ s% |8 ?8 f+ z0 G- @
3 Safety Aspects of Cell Culture 0 \" u4 u* O' N* Q4 Sourcing of Cell Lines" a+ Y( W; D+ |, V" d* ^
5 Cell Types & Culture Characteristics 5 M' J7 R4 v1 `3 H! e; r" K6 _/ r6 The Cell Environment (including types of culture medium) ) ?7 ?* d3 a' ^% }2 d S7 Cryopreservation and Storage of Cells 9 P9 l9 k0 J5 w, x3 v8 Good Cell Banking Practices2 h: _# o( y& s9 X: ~
9 Quality Control Considerations in Cell Culture 1 m$ Y5 m$ y" {10 Authentication of Cell Lines5 X9 Q: ^. h& }$ u4 n4 u u5 x
11 Alternative Culture Systems. j$ Z7 d' U, y4 g
12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture- Q' w0 }: H5 e' ~/ @
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Protocols Title( G8 C, I1 Y' m I) P' X
1 Aseptic Technique and Good Cell Culture Practice 2 |# Y" t# ^6 {3 t$ L# W2 Resuscitation of Frozen Cell Lines. y2 P6 [$ y$ _1 e+ ?/ w# P
3 Subculture of Adherent Cell Lines / d, c4 q+ n( |4 Subculture of Semi-Adherent Cell Lines$ M: g6 C- ~+ F2 K& v |7 w
5 Subculture of Suspension Cell Lines g+ Q$ P8 p3 ^7 r
6 Cell Quantification1 y0 R# h' A4 W+ F! W
7 Cryopreservation of Cell Lines" Y$ P) P! u& A
8 Testing for Bacteria and Fungi in Cell Cultures7 d; R5 l( i: p
9 Testing for Mycoplasma by Culture Isolation ' O5 e1 u3 K5 j r10 Testing for Mycoplasma by Indirect DNA Stain % G5 p6 M! Z9 [ 0 `1 ?$ k6 w- C0 N0 Q/ FGlossary of Terms/ g5 {% o9 I; o( r# R4 G" h+ \7 F" T 作者: tjbone 时间: 2011-9-1 15:25
学习谢谢了！！！！！作者: langziforever 时间: 2011-9-1 15:52
非常感谢！作者: hormone008 时间: 2011-9-3 11:42
good6 N' e4 Q: q r, R/ g, _( @( L
word: \ \1 Q- Q' y" ^6 a5 C, A
thanks7 D5 F. k) j0 ]( S3 M5 V# { G 作者: cc1986 时间: 2011-9-4 12:36
