标题: One protein, two pools [打印本页] 作者: 杨柳 时间: 2009-3-6 08:14 标题: One protein, two pools
. , a$ @) O! v6 `" f* @ $ Y7 R+ b; L0 L0 @# e o. aCofractionation experiments showed that ?-catenin that interacts with cadherin is in a heterodimer with -catenin, but Wnt signaling induces a monomeric form of ?-catenin that interacts with a transcription factor complex. After enriching for cadherins, ?-catenin can be detected using antibodies to either NH2-terminal or COOH-terminal regions; but in the cadherin-free fraction only the NH2-terminal antibody binds. The team hypothesizes that the ?-catenin protein is folded back on itself in its monomeric form and, as such, is available for transcription complex binding but not for adhesion duties. 1 {1 Q1 ^# }" W) Q . X2 S6 J" X' W; h' T3 d% pGottardi speculates that a post-translational modification is responsible for the conformational change but does not yet know what that modification is. Furthermore, she thinks this sort of molecular segregation may be a common mechanism cells use to allow one protein to do multiple jobs, without the more common function soaking up all the protein required for a temporally regulated one.(The cytoplasmic protein ?-catenin is ess)作者: 昕昕 时间: 2015-5-28 13:27