5 J- {& @* ^( U" h& I+ e1 Y6 K最后研究者Benning说道,CHT7蛋白将为癌症领域研究开辟一片新的天地,这种分子开关会促使有机体无限生长,后期研究中我们或许会通过CHT7蛋白来研究癌症扩散及肿瘤无限增殖的分子机制。(生物谷Bioon.com) i6 S5 c! E7 L. V% d, p' ~0 f6 v* ?- Y8 a* g$ k
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doi:10.1073/pnas.1414567111 ! k+ B( D0 b" Z9 J0 q- Y! YPMC: % \. F9 z% s: H' IPMID: ) _( L" q- w: \0 u$ t9 E3 C5 z, O# `5 @& T9 r' y
The protein Compromised Hydrolysis of Triacylglycerols 7 (CHT7) acts as a repressor of cellular quiescence in Chlamydomonas 9 [2 I8 O7 H9 w3 y / D4 q, G9 ^; u4 `) K, o" N4 e- QChia-Hong Tsaia,b, Jaruswan Warakanontb, Tomomi Takeuchic, Barb B. Searsb, Eric R. Moelleringc,1, and Christoph Benningc,2 + m7 B. W1 n: A' V% X M; Z' E$ L " l9 _8 I2 J. `Microalgae are prolific photosynthetic organisms that have the potential to sustainably produce high-value chemical feedstocks. However, an industry based on microalgal biomass still is faced with challenges. For example, microalgae tend to accumulate valuable compounds, such as triacylglycerols, only under stress conditions that limit growth. To investigate the fundamental mechanisms at the base of this conundrum—the inverse relationship between biomass production and storage compound accumulation—we applied a combination of cell biological and genetic approaches. Conceptually, nutrient deprivation, which commonly is used to induce the accumulation of triacylglycerol in microalgae, leads to a state of cellular quiescence defined by a halt of cell divisions that is reversible upon nutrient resupply. To identify factors that govern cellular quiescence, we screened for mutants of the model alga Chlamydomonas reinhardtii that, in contrast to wild-type cells placed under conditions of nitrogen deprivation, were unable to degrade triacylglycerols following nitrogen resupply. One of the mutants described here in detail, compromised hydrolysis of triacylglycerols 7 (cht7), was severely impaired in regrowth following removal of different conditions inducing cellular quiescence. The mutant carries a deletion affecting four genes, only one of which rescued the quiescence phenotype when reintroduced. It encodes a protein with similarity to mammalian and plant DNA binding proteins. Comparison of transcriptomes indicated a partial derepression of quiescence-related transcriptional programs in the mutant under conditions favorable to growth. Thus, CHT7 likely is a repressor of cellular quiescence and provides a possible target for the engineering of high-biomass/high-triacylglycerol microalgae.作者: 齐学康 时间: 2014-10-21 11:13