naturalkillerce根据下面的英文新闻报道，作如下编译：+ E1 V8 X8 M' u/ o
当在盘碟中培养干细胞时，这些干细胞天然地附着在饲养细胞上。多年以来，科学家们一直认为这种附着之所以发生，是因为饲养细胞作为一种支持系统给干细胞提供必需的营养物。0 {+ I( {! \; M" ]% a
但是，在一项新的研究中，研究人员成功地利用死亡的或者说固定的饲养细胞来培养干细胞，相关研究结果发表在Journal of Materials Chemistry B期刊上，文章标题为“Chemically fixed autologous feeder cell-derived niche for human induced pluripotent stem cell culture”。这一发现挑战了现有理论，即饲养细胞给体外培养的干细胞提供营养物。! A a) y, N. T* X3 o6 w
在这项研究中，在活的干细胞放置在盘碟中之前，饲养细胞先通过化学固定的方法固定在盘碟中。这种处理方法虽然让这些饲养细胞在外观上保持相同，但是已经将它们杀死。 2 g0 I. T/ U2 w% j# G5 G+ _ 当加入干细胞后，尽管这些饲养细胞已经死掉，但是干细胞仍然附着在它们上面，并且成功地生长。+ k2 I, Y& W5 r- }- O. f% {) x
这一发现提供一种更加简单且更为节省成本的干细胞培养方法。这是因为在干细胞培养过程中，饲养细胞并不需要保持活性，因此人们能够在室温下储存它们，花费比较少的时间来培养它们。 ' K; }4 x- j' p" e8 ?1 { 1 v8 {2 {5 u7 {6 A4 uDead feeder cells support stem cell growth 6 y4 p% y: c# w9 S0 e9 e, dStem cells naturally cling to feeder cells as they grow in petri dishes. Scientists have thought for years that this attachment occurs because feeder cells serve as a support system, providing stems cells with essential nutrients.% H7 o( F$ {$ w) {1 T B7 V7 \
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But a new study that successfully grew stem cells with dead, or fixed, feeder cells suggests otherwise. & G8 G2 [7 ~* fThe discovery, described in the Journal of Materials Chemistry B, challenges the theory that feeder cells provide nutrients to growing stem cells. It also means that the relationship between the two cells is superficial, according to Binata Joddar, Ph.D., a biomedical engineer at The University of Texas at El Paso (UTEP). % J2 f+ v( K" k- H+ [# I+ O: t# w* v9 G/ a* ~& d
"We've proved an important phenomenon," said Joddar, who runs UTEP's Inspired Materials and Stem-Cell Based Tissue Engineering Lab. "And it suggests that these feeder cells, which are difficult to grow, may not be important at all for stem cell growth." 5 J5 m) y0 q6 f2 D3 `& D 6 B4 ^' g& }$ s) f4 }- s4 c( PIn the study, feeder cells were chemically fixed before living stem cells were placed in the same dish. Like organs that are preserved with formaldehyde, this kept the feeder cells' physical appearance the same, but essentially killed them.Even though the feeder cells were dead, the stem cells still latched on and grew successfully.% \9 L u' k& V0 L0 ~
The discovery offers a simpler and more cost-effective way to grow stem cells, which has proved difficult over the years. R3 s9 o0 M/ w; W' x. T
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"Because feeder cells don't need to stay alive in the process, we can store them at room temperature and spend less time cultivating them," Joddar said.; ]; a- k3 M: `0 E
Joddar believes the finding suggests that stem cells may only like the "topology" of feeder cells. " B; \. V. J7 ]. B' ~- m# P"This makes me think that we use a nanomanufacturing approach to grow stem cells," she said. "We could mimic feeder cells' nanotopology with 3-D printing techniques and skip using feeder cells altogether in the future." 2 ?* b" |4 x4 c- t3 ihttp://phys.org/news/2015-04-dead-feeder-cells-stem-cell.html 7 j8 |6 w' c% b作者: naturalkillerce 时间: 2015-4-29 14:13
回复 FreeCell 的帖子- e$ S v3 P0 |+ H- c
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这篇文章的主要意义在于：干细胞的培养不在于饲养细胞的死亡或存货，而在于饲养细胞经过化学交联剂固定后仍然提供拓扑结构，从而让干细胞通过附着这种结构而生长。如果饲养细胞只是死亡，而不能提供这样的拓扑结构，还能否让干细胞继续生长？, \5 l1 d3 v3 A% k9 V0 P
我觉得作者的话是蛮有道理的："This makes me think that we use a nanomanufacturing approach to grow stem cells," she said. "We could mimic feeder cells' nanotopology with 3-D printing techniques and skip using feeder cells altogether in the future." : \4 U2 ~0 ]. S# G. W2 H不要动则炒冷饭来加以否定，哪有这么多创造性的研究啊？只要有新意，仍然具有意义。作者: hyde 时间: 2015-5-1 12:33
回复 naturalkillerce 的帖子 ) h% e) z% Y/ R0 C1 Y # v" \8 I+ k$ L+ b$ o, y"This makes me think that we use a nanomanufacturing approach to grow stem cells," she said. "We could mimic feeder cells' nanotopology with 3-D printing techniques and skip using feeder cells altogether in the future."——为赋新词强说愁
