多能干细胞基因组编辑：方法，误区和解决方案

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& H! [. E. Q9 X- |) u1月6日，Cell子刊《Cell Stem Cell》在线发表了来自哈佛大学和麻省总医院研究人员的一篇综述文章，题为“Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions”。
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Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions* m, X" `; [5 f# ?4 `+ @% y5 M* \/ T
Summary: Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.* w4 a/ _$ t' t# I$ Z
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