请问ips培养中Y-27632的作用是什么

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Y-27632为ROCK inhibitor,防止细胞凋亡的。
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Rho激酶（Rho associated kinase, ROCK）,参与细胞信号通路。ROCK inhibitor为ROCK 抑制剂。
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' ?' Y) e  e& x9 y/ W1 Q7 S关于ROCKi的详细介绍，给你找了一篇文文献。A ROCK inhibitor permits survival of dissociated human embryonic stem cells.
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这个我也不是很知道啦，不好意思。问了下同事，说的是：因为刚开始，还没有活化，ROCK容易启动，导致凋亡。等细胞长起来了，就可加可不加了。6 z7 g- j( Y+ x9 S

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# V) n* r! i3 U' c0 U8 _5 M) \" D再来点英文的补充：
5 a7 P6 O/ }6 C, C7 RRho-associated protein kinase (ROCK) is a protein involved in the regulation of+ x4 |0 O* h! L1 m. I1 d0 `
several forms of cellular behaviour. Among these is the regulation of apoptosis.3 {- _+ s1 B0 i8 ?+ g: v( {: g" i1 Q
When a cell is preparing to undergo apoptosis, caspase 3 will cleave the5 l4 b2 C5 Q1 d4 G" i# m$ _
autoinhibiting C-terminal of the ROCK protein and the cell will perform a  ?% v- R8 h. A. n$ g- S4 c
cytoskeletal change known as membrane blebbing. In order to prevent apoptosis, D# d$ @$ f; C: R) i3 ^& c5 a* S# b- |
ROCK inhibitors (ROCKi) are added to stem cell cultures preventing the membrane
7 {0 ~/ j' B3 q2 H# \% lblebbing and increasing cell survival by 20-30%. The use of ROCKi is universal and2 k+ W3 Q, E$ w/ T" l/ {
most scientists use this inhibitor to prevent cell death especially during single-cell1 f: B5 Y1 M3 l' ]6 a
passaging and clonal assays. 3 f% ]- i. A! ^& k: W. R2 [
Benefits of not using ROCKi
* z3 d. z- F# {0 M* i# D, s• Less is always more when culturing stem cells, fewer components added is beneficial both from an economical and a functional standpoint.2 w3 U& n( b7 P- j
• There is a concern throughout the cell culture community that the ROCKi might affect the morphology and genetic stability of the cells. ROCKi promotes the survival of “bad” cells that would otherwise have undergone apoptosis which could lead to complications. These claims have/ X1 T3 s! o; _* B; s: m
not yet been validated but studies are underway and we believe that this is a benefit even today.
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- B8 h/ h; T' _; H, `& x其实现在已经有很多ROCKi-free的体系了:victory: