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谭铮简介:- h7 N( U% H! M1 s* w) a3 M
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谭铮,博士。中科院动物所生物膜与膜生物工程国家重点实验室研究员。# g5 N3 u. m- s
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1978-1982 武汉大学 生物系生物化学,学士7 V5 x8 \7 P' h0 @; Y
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1982-1985 武汉大学 生物系生物物理,硕士" Q0 q, |$ M; h- f
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1985-1989 北京 中国科学院 动物研究所生物化学,博士6 o- o" q3 \7 k9 j) R
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1989-1991 北京 中国科学院 动物研究所 助理研究员
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5 ], T$ X7 b2 c& U1991-1993 美国北卡州立大学 植物系 博士后% d) Z2 s/ ^( k+ Q( z" p) P
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1993-1996 美国国立卫生研究院环境健康科学研究所 Visiting Fellow
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1997-1998 广州华南理工大学食品与生物工程学院 副教授/ _" f$ @5 [! \- g$ t$ s3 Q
. K( n4 S) |& e3 ?1999-2005 武汉大学 生命科学院 教授,博导,长江计划特聘教授
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: U. X( `+ ]" |2005- 中科院动物所生物膜与膜生物工程国家重点实验室 研究员
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主要研究领域:
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: s" z" H' x# l [6 s6 ~细胞衰老的机制的研究,主要采用生物化学,生物物理和细胞生物学的手段研究染色体端粒DNA结构与功能,端粒损伤和复制机理,端粒结合蛋白,端粒细胞分裂控制与细胞复制性衰老。! {2 V% }3 L% }* P( J9 D
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近年来主要发表文章:
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[1] Zheng, K. W., Chen, Z., Hao, Y. H. and Tan, Z. (2009) Molecular crowding creates an essential environment for the formation of stable G-quadruplexes in long double-stranded DNA. Nucleic Acids Res., (In press).
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7 ]/ y* V l0 H[2] Chen, Z., Zheng, K. W., Hao, Y. H. and Tan, Z. (2009) Reduced or diminished stabilization of telomere G-quadruplex and inhibition of telomerase by small chemical ligands under molecular crowding condition. J Am Chem Soc 131, 10430-10438.% R _- g- G) ?" }4 B/ C( P* R
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[3] Tang, J., Kan, Z. Y., Yao, Y., Wang, Q., Hao, Y. H. and Tan, Z. (2008) G-quadruplex preferentially forms at the very 3' end of vertebrate telomeric DNA. Nucleic Acids Res 36(4): 1200-8.
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[4] Yuan, B. F., Zhuang, X. Y., Hao, Y. H. and Tan, Z. (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb)(48): 6600-2.+ l% `; Y# i& g4 F( L3 B5 n
1 @8 q5 d' h5 t0 `+ k( s0 N' e[5] Wang, F., Zhao, Y., Hao, Y. and Tan, Z. (2008) Identification of low-abundance alternatively spliced mRNA variants by exon exclusive reverse transcriptase polymerase chain reaction. Anal Biochem 383: 307-10.
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[6] Xue, Y., Kan, Z. Y., Wang, Q., Yao, Y., Liu, J., Hao, Y. H. and Tan, Z. (2007) Human telomeric DNA forms parallel-stranded intramolecular G-quadruplex in K+ solution under molecular crowding condition. J Am Chem Soc 129(36): 11185-91.
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[7] Yao, Y., Wang, Q., Hao, Y. H. and Tan, Z. (2007) An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small molecules. Nucleic Acids Res 35(9): e68.
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, Y3 N) _9 i! y' L" @, [1 b[8] Kan, Z. Y., Lin, Y., Wang, F., Zhuang, X. Y., Zhao, Y., Pang, D. W., Hao, Y. H. and Tan, Z. (2007) G-quadruplex formation in human telomeric (TTAGGG)4 sequence with complementary strand in close vicinity under molecularly crowded condition. Nucleic Acids Res 35(11): 3646-53. ?0 I/ x: y3 X! u/ a3 G
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[9] Zhuang, X. Y., Tang, J., Hao, Y. H. and Tan, Z. (2007) Fast detection of quadruplex structure in DNA by the intrinsic fluorescence of a single-stranded DNA binding protein. J Mol Recognit 20(5): 386-91.( X$ K. Q; a1 x% k# \
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[10] Yuan, B. F., Xue, Y., Luo, M., Hao, Y. H. and Tan, Z. (2007) Two DNAzymes targeting the telomerase mRNA with large difference in Mg2+ concentration for maximal catalytic activity. Int J Biochem Cell Biol 39(6): 1119-29.4 l" ^ }# A, t, k& Y
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[11] Kan, Z. Y., Yao, Y., Wang, P., Li, X. H., Hao, Y. H. and Tan, Z. (2006) Molecular crowding induces telomere G-quadruplex formation under salt-deficient conditions and enhances its competition with duplex formation. Angew Chem Int Ed Engl 45(10): 1629-32.
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[12] Zhao, Y., Zeng, Z. X., Kan, Z. Y., Hao, Y. H. and Tan, Z. (2005) The folding and unfolding kinetics of the i-motif structure formed by the C-rich strand of human telomere DNA. Chembiochem 6(11): 1957-60.
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i; T' B: [$ A[13] Zeng, Z. X., Zhao, Y., Hao, Y. H. and Tan, Z. (2005) Tetraplex formation of surface-immobilized human telomere sequence probed by surface plasmon resonance using single-stranded DNA binding protein. J Mol Recognit 18(3): 267-71.
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[14] Shao, L., Li, Q. H., Wang, J. and Tan, Z. (2005) Fragmentation and rapid shortening of telomere in HeLa cells in the early phase of hydroxyl radical-induced apoptosis. Cancer Biol Ther 4(3): 336-41.! E; P: y4 }; B5 h
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[15] Li, W. G., Li, Q. H. and Tan, Z. (2005) Epigallocatechin gallate induces telomere fragmentation in HeLa and 293 but not in MRC-5 cells. Life Sci 76(15): 1735-46.2 k( h' E4 {6 {- B, ]/ E0 O, s
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[16] Li, W. G., Li, Q. H. and Tan, Z. (2005) Detection of telomere damage as a result of strand breaks in telomeric and subtelomeric DNA. Electrophoresis 26(3): 533-6.
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* l5 O6 [) K" j4 `: d' B; n[17] Zhao, Y., Kan, Z. Y., Zeng, Z. X., Hao, Y. H., Chen, H. and Tan, Z. (2004) Determining the folding and unfolding rate constants of nucleic acids by biosensor. Application to telomere G-quadruplex. J Am Chem Soc 126(41): 13255-64.
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( `# y4 s0 r$ H' ~( j[18] Yuan, B. F., Hao, Y. H. and Tan, Z. (2004) Universal sensing strategy for the detection of nucleic acid targets by optical biosensor based on surface plasmon resonance. Clin Chem 50(6): 1057-60." p& S- N: u( {2 m# Q# m- P( |
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[19] Shao, L., Li, Q. H. and Tan, Z. (2004) L-carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts. Biochem Biophys Res Commun 324(2): 931-6.( d9 g/ E3 @- U" ]2 k
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[20] Hao, Y. H. and Tan, Z. (2002) The generation of long telomere overhangs in human cells: a model and its implication. Bioinformatics 18(5): 666-71.
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- u3 \. `) K# ~6 M; R( M- z4 X[21] Tan, Z. (2001) Simulated shortening of proliferation-restricting telomeres during clonal proliferation and senescence of human cells. Exp Gerontol 36(1): 89-97.+ R' E. M4 T" j1 F5 a
) w' L+ R, ^9 w5 D. x6 C4 U$ X# N[22] Hao, Y. H. and Tan, Z. (2001) Telomeres at the chromosome X(p) might be critical in limiting the proliferative potential of human cells. Exp Gerontol 36(10): 1639-47. |
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