求1988年Nature Biotechnology文章一篇

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Nature Biotechnology 16, 642 - 646 (1998)
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+ m, G1 T+ R, t+ j7 P2 mTrasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cellsJose B. Cibelli1, ,2, Stevem L. Stice2, Paul J. Golueke2, Jeff J. Kane2, Joseph Jerry1, Cathy Blackwell2, F. Abel Ponce de León2, ,3 & James M. Robl1, ,2, ,** a- p! J8 C8 Q/ p

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+ v& b' _- o/ Z- N) |4 nAbstract  We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endo-derm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
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全文链接+ k; W1 f- }$ O5 J
http://www.nature.com/nbt/journal/v16/n7/abs/nbt0798-642.html

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Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells* {7 @2 ?' `) i# D
1998

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