两种支架上的牙周膜干细胞的生物相容性和成骨能力

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J Biomater Sci Polym Ed. 2010 Jun 16. [Epub ahead of print]
0 m! B4 i# _6 Q% y) @2 F5 zBiocompatibility and Osteogenic Capacity of Periodontal Ligament Stem Cells on nHAC/PLA and HA/TCP Scaffolds.
, V9 |, O( B& c7 \3 v3 H1 D! MHe H, Yu J, Cao J, E L, Wang D, Zhang H, Liu H.( T$ i+ ?# r2 G7 y9 a% d
Abstract
" s/ w  B% A( \2 C8 N$ q3 }This study investigated the effects of a newly-developed scaffold, nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA), on the attachment, proliferation and osteogenic capability of dog periodontal ligament stem cells (PDLSCs) in vitro and in vivo. Hydroxyapatite/tricalcium phosphate (HA/TCP), a commonly used bone substitute, was used as a positive control. PDLSCs isolated from dog molar were incubated in an osteogenic medium to evaluate their osteogenic differentiation in vitro, and then seeded onto nHAC/PLA and HA/TCP scaffolds. In vitro cell attachment, proliferation and differentiation were assessed by scanning electron microscopy (SEM), cell counting, 3-[4,5-dimethythiazol-2-yl]-5-[3-carboxy-phenyl]-2-[4-sulfophenyl]-2H-tetrazolium and alkaline phosphate activity, and reverse transcription-polymerase chain reaction, respectively. Finally, the constructs were implanted subcutaneously into dogs to investigate their osteogenic capacity. After osteogenic induction for 21 days, PDLSCs differentiated into osteogenic lineage, as indicated by the expressions of osteoblastic differentiation genes CoL-I, OCN and OPN mRNA, and the formation of mineral deposits. When seeded onto scaffolds, the cells attached and spread well, and retained their osteogenic phenotypes on both scaffolds. Comparatively, cell number and proliferative viability on nHAC/PLA constructs were greater than those on HA/TCP constructs (P<0.05). Histological results showed that new bone and osteoid was formed in both groups, and histomorphometric analysis demonstrated that the amount of newly formed bone in the nHAC/PLA group was higher than that in the HA/TCP group (P<0.05). This study suggests that nHAC/PLA can be used as a potent scaffold for alveolar bone regeneration.
: D/ g( Z7 M( CPMID: 20557694 [PubMed - as supplied by publisher]. w7 I" i$ [9 ~3 a/ r

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J Biomater Sci Polym Ed. 2010-6-16  付印前电子版2 u2 W' z* @$ I/ p; @' u
纳米羟基磷灰石/胶原/聚乳酸支架和羟基磷灰石/ 磷酸三钙支架上的牙周膜干细胞的生物相容性和成骨能力
! A7 h8 u- ~8 KHe H, Yu J, Cao J, E L, Wang D, Zhang H, Liu H.
; N$ z1 j2 E8 w: q摘要
1 k& ~- z- n. x7 B9 E本研究从体内外狗牙周膜干细胞的附着、增殖和成骨能力方面，研究了一种新的支架—纳米羟基磷灰石/胶原/聚乳酸支架的作用。以一种常用的骨替代物——羟基磷灰石/ 磷酸三钙支架作为阳性对照组。成骨诱导培养基培养从狗磨牙分离的牙周膜干细胞以使其体外成骨分化，然后将分化了的细胞分别接种到纳米羟基磷灰石/胶原/聚乳酸支架和羟基磷灰石/ 磷酸三钙支架上。通过扫描电镜，细胞计数，3-[4,5-dimethythiazol-2-yl]-5-[3-carboxy-phenyl]-2-[4-sulfophenyl]-2H-tetrazolium，碱性磷酸酶活性和逆转录聚合酶链反应分别检测体外细胞的附着、增殖和分化。最后，将复合物移植入狗皮下以研究其成骨能力。成骨诱导21天后的牙周膜干细胞表达成骨细胞分化基因胶原蛋白-I，骨钙蛋白和骨桥蛋白的mRNA并形成矿化结节，表明其分化成成骨系细胞。接种到支架的细胞附着、扩散良好，两种支架上的细胞均保持了成骨表型。与羟基磷灰石/磷酸三钙支架相比，纳米羟基磷灰石/胶原/聚乳酸支架上的细胞数量更多，增殖活力更好(P<0.05)。组织学结果显示两组中均有新骨和骨样结构形成，组织形态测量分析证明纳米羟基磷灰石/胶原/聚乳酸支架中新形成的骨量比羟基磷灰石/磷酸三钙支架中的多(P<0.05)。本研究说明纳米羟基磷灰石/胶原/聚乳酸支架有望用于牙槽骨再生。
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外文翻译要求和原则“信、达、雅”。“信”，要使译文准确无误，忠于原文，如实恰当地运用现代汉语把原文翻译出来。“达”，就是要通顺畅达，要使译文符合现代汉语的语法及用语习惯，字通句顺，没有语病。“雅”就是指译文要优美自然，要使译文生动、形象，完美地表达原文的写作风格。
1 Z' y$ y" Q' E刚刚查到的，在前辈的指导下，我做到了“信”，我要向下一个目标冲刺了。
2 S- z5 y2 r8 P: ]3 E8 t修正后译文如下：
1 ~; K) Q. U8 RJ Biomater Sci Polym Ed. 2010-6-16  付印前电子版
7 K/ K5 S) @' ]; J$ I0 {6 X牙周膜干细胞在纳米羟基磷灰石/胶原/聚乳酸支架和羟基磷灰石/ 磷酸三钙支架上的生物相容性和成骨能力比较
- a' `2 G; W' K6 U9 aHe H, Yu J, Cao J, E L, Wang D, Zhang H, Liu H.
7 M$ ?2 r: c. u# |: J6 U摘要
  Y1 w+ X6 R+ v# M9 Z+ n本研究对一种新的支架—纳米羟基磷灰石/胶原/聚乳酸支架在狗牙周膜干细胞的附着、增殖和成骨能力上的作用进行了体内和体外检验。以一种常用的骨替代物——羟基磷灰石/ 磷酸三钙支架作为阳性对照组。成骨诱导培养基培养从狗磨牙分离的牙周膜干细胞以使其体外成骨分化，然后将分化了的细胞分别接种到纳米羟基磷灰石/胶原/聚乳酸支架和羟基磷灰石/ 磷酸三钙支架上。通过扫描电镜，细胞计数，3-[4,5-dimethythiazol-2-yl]-5-[3-carboxy-phenyl]-2-[4-sulfophenyl]-2H-tetrazolium，碱性磷酸酶活性和逆转录聚合酶链反应分别检测体外细胞的附着、增殖和分化。最后，将复合物移植入狗皮下以研究其成骨能力。成骨诱导21天后的牙周膜干细胞表达成骨细胞分化基因胶原蛋白-I，骨钙蛋白和骨桥蛋白的mRNA并形成矿化结节，表明其分化成成骨系细胞。接种到支架的细胞附着、扩散良好，在两种支架上均保持了成骨表型。与羟基磷灰石/磷酸三钙支架相比，纳米羟基磷灰石/胶原/聚乳酸支架上的细胞数量更多，增殖活力更强(P<0.05)。组织学结果显示两组中均有新骨和骨样结构形成，而组织形态测量分析则表明纳米羟基磷灰石/胶原/聚乳酸支架中新形成的骨量高于羟基磷灰石/磷酸三钙支架中的骨量(P<0.05)。本研究表明，纳米羟基磷灰石/胶原/聚乳酸支架可以作为一种牙槽骨再生的高效能支架。
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以前从未想过将potent译成 高效能，学习了