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本帖最后由 细胞海洋 于 2011-6-1 10:59 编辑 , }& ?# |; G3 {7 z
6 e6 K6 P" P% G N% k不知道这本有没有上传过，搜索了一下似乎没有结果，还有这个书应该是hualin840518版主所说的Humana Press出版社出版《生物技术方法》Methods in Biotechnology中的一册吧，请版主鉴定一下，如有误，请麻烦更正，谢谢。
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Directed Evolution Library Creation( J$ H6 z% r; }+ T( Z6 v) m0 i
Methods and Protocols8 Z: y5 q4 t5 C
Series: Methods in Molecular Biology, Vol. 231; S/ ]8 y" g2 [ U' m
Arnold, Frances H.; Georgiou, George (Eds.)
+ u' ^7 T/ r1 {, U2 L+ K2003, ISBN 978-1-58829-285-8, Hardcover
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A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes.
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8 D" ]3 C1 A+ w( kDirected Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.9 B8 ^4 K5 L! O( }+ v" o
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发表于 2011-6-1 10:56
