|
- 积分
- 17665
- 威望
- 17665
- 包包
- 23467
|
Choreographing pluripotency and cell fate with transcription factors
/ W, d) g O0 |1 PGonzales KA, Ng HH., O2 F$ j$ ?, Q Y, h
Abstract4 v9 H% }- a& e
The cellular identity of both pluripotent and differentiated cells is defined by the concerted interplay of transcriptional factors as well as other modulators such as epigenetic and signaling mediators. Therefore, the manipulation of a cell's transcriptional network directly facilitates inter-conversion between cellular identities. Understanding the molecular regulation of cell fate changes, including those involved in pluripotency, is crucial in realizing the practical potential of pluripotent and induced cell types. Here we review the advancements in the role of transcription factors in pluripotency, as well as in the conversion between and within pluripotent and somatic cell types., G3 {$ k+ c$ p$ ]6 e5 g0 t% f
( G/ f/ I2 J% l- a0 y$ E- {
http://www.ncbi.nlm.nih.gov/pubmed/21722763
# O% e; a9 c" q |( u+ q& U; f8 |
! b- F3 l5 L- Q' ^1 m. c( s
Stem cell genome‐to‐systems biology5 N/ _$ m. p9 J$ W& J4 z! |
$ j. s; i1 X% _* I) lStem cells are capable of extended proliferation and concomitantly differentiating into a plethora of specialized cell types that render them apropos for their usage as a form of regenerative medicine for cell replacement therapies. The molecular processes that underlie the ability for stem cells to self‐renew and differentiate have been intriguing, and elucidating the intricacies within the genome is pertinent to enhance our understanding of stem cells. Systems biology is emerging as a crucial field in the study of the sophisticated nature of stem cells, through the adoption of multidisciplinary approaches which couple high‐throughput experimental techniques with computational and mathematical analysis. This allows for the determination of the molecular constituents that govern stem cell characteristics and conjointly with functional validations via genetic perturbation and protein location binding analysis necessitate the construction of the complex transcriptional regulatory network. With the elucidation of protein–protein interaction, protein–DNA regulation, microRNA involvement as well as the epigenetic modifications, it is possible to comprehend the defining features of stem cells at the system level. WIREs Syst Biol Med 2011 DOI: 10.1002/wsbm.151
2 [! V R r% M/ w4 |9 g6 v3 d
' X( K8 U. C$ `. p& z1 Z1 @' vhttp://wires.wiley.com/WileyCDA/WiresArticle/wisId-WSBM151.html
7 R- b: t: _$ A/ y$ i
. Y! L& T- K# R- }* F k, b4 n+ ]$ Q, d7 E0 H9 s! w7 P) N3 p! D
|
|
发表于 2011-10-4 20:28
