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Selectins with a catch

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发表于 2009-3-6 00:47 |显示全部帖子
Zhu/Macmillan
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Not content simply with a good fit, evolution has apparently designed adhesion molecules that stick optimally only when tugged upon. This property, say Bryan Marshall and Cheng Zhu (Georgia Institute of Technology, Atlanta, GA), and Tadayuki Yago and Rodger McEver (University of Oklahoma, Oklahoma City, OK), and colleagues, may stop leukocytes and platelets from lingering in stagnant backwaters in the blood where there is little or no flow.+ H9 t9 V0 j0 Y+ {- h
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Blood is a medium dominated by flow and shear forces. "If you think flow is disruptive, then greater flow should make cells roll faster," says Zhu. "That's the case at the high end, but at the low end it reverses."  ]/ `: l0 s/ B% |/ z$ T6 S
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The Georgia Tech group found that this effect could be attributed to the behavior of individual adhesion molecules such as P-selectin and its partner P-selectin glycoprotein ligand-1 (PSGL-1). Leukocytes roll along vessel walls when their PSGL-1 binds to the P-selectin found on activated endothelial cells.( p+ S. d% `+ O% p, Z" e

* j5 ^  v. U3 Q* f6 K1 v! |6 |. W. HAt high shear forces, increasing shear leads to decreasing binding lifetime. But the Georgia Tech group found that this "slip" mode was reversed at lower shear forces. In this "catch" mode, found only below a certain limit of shear force, binding lifetime increased with increasing shear force.- g0 P* I! f6 ~0 b' Q: h

/ Y  A- C1 Q: O6 hFor the individual binding events measured, the catch mode shear forces were small〞as small as those produced by a few molecular motors. But Zhu feels that the catch mode is relevant in the real world, where the larger shear forces are distributed across many binding events per cell.: m) U( p- o. ^
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The crystal structure of the binding complex does not point to an obvious catch mechanism, although molecular dynamics simulations may yield clues. Zhu is also interested in exploring the binding energy landscapes. Any bound state is a kinetic trap, so perhaps the force both raises the bottom of this energy well and, even more, elevates the energy barrier blocking escape. Or force might make a short lifetime exit path inaccessible. Whatever the mechanism, the product is a cell that inspects closely only when it has a moving target.
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4 v) u. p/ N( M. b3 T- R9 V0 n3 qMarshall, B.T., et al. 2003. Nature. 423:190–193.(At low forces, adhesion lifetimes increa)

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发表于 2015-5-24 12:26 |显示全部帖子
你还想说什么啊....  

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发表于 2015-5-28 20:10 |显示全部帖子
@,@..是什么意思呀?  

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发表于 2015-6-19 11:42 |显示全部帖子
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嘿嘿......哈哈......呵呵.....哟~呼  

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发表于 2015-6-19 20:54 |显示全部帖子
真是佩服得六体投地啊  

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发表于 2015-6-28 20:10 |显示全部帖子
顶顶更健康,越顶吃的越香。  

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发表于 2015-7-5 22:10 |显示全部帖子
转基因动物

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发表于 2015-7-7 18:31 |显示全部帖子
经过你的指点 我还是没找到在哪 ~~~  

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发表于 2015-8-8 16:51 |显示全部帖子
加油站加油  

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发表于 2015-8-31 14:10 |显示全部帖子
挺好啊  
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