Vc与bFGF的联合作用解决脂肪干细胞培养的问题！！！

Yorkshining

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       论坛里经常看到大家为脂肪干细胞的培养而苦恼，最近在《In Vitro Cell.Dev.Biol.—Animal》发表了一篇关于利用Vc和bFGF的联合作用，能够有效的刺激小鼠脂肪干细胞的增殖，分化，分泌，延缓细胞衰老和细胞转化的效果，细胞可传至30代以上，这种培养体系也许也有利于其它物种的脂肪干细胞或间充质干细胞的培养，希望对奋斗在与间充质干细胞相关的工作的各位有所帮助，以下是文章摘要！
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. c- [9 o. f$ V( W  YIn Vitro Cell Dev Biol Anim. 2013 Aug 16. [Epub ahead of print]
  W8 Y: S8 h6 w: v9 tEffects of vitamin C on characteristics retaining of in vitro-cultured mouse adipose-derived stem cells.
0 i" ~! b( ^$ qWei C, Liu X, Tao J, Wu R, Zhang P, Bian Y, Li Y, Fang F, Zhang Y.
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Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, 230036, China.# n  V9 w1 p" T/ C0 N5 f  Q
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Abstract
9 s8 b  M. c% R1 C2 D$ p$ mAdipose-derived stem cells (ADSCs), a subset of mesenchymal stem cells, have promising potential for regenerative medicine applications. However, the efficient culture of mouse adipose-derived stem cells (mADSCs) is complicated or impracticable and many properties of mADSCs are still unknown. Here, we report that vitamin C (Vc) is available for the long-term culture of mADSCs in vitro. Compared with that cultured without Vc, mADSCs growing in Vc-added media proliferate faster. The occurrence of replicative senescence and transformation of Vc-treated mADSCs is also postponed. Vc also enhanced the secretory activity of collagen and adipogenic differentiation ability of mADSCs. Moreover, the expression of CD44, Sca-1, and CD105 is higher in Vc-treated mADSCs than nontreated ones. We also found that genes related to proliferation, secretion, and pluripotency are all promoted in Vc-treated mADSCs. However, the adipogenesis ability and expression of CD44, Sca-1, and CD105 decreased when passage increased from very low passages, in parallel to the downregulation of closed-related gene expression. Our results indicate that Vc is essential for the maintenance of original properties of mADSCs in vitro; additional insights into the function of Vc on mADSCs are provided. Furthermore, as the passage increased in six passages, the characteristics of mADSCs with Vc addition were also revealed.

Andyhigh

顶一个···

cartoonyang

鼎！！！

xuyang0317

帮你顶一个，这篇文章感觉真的不错，有机会会尝试下

yaojing929

请问诱导脂肪干细胞向心肌细胞的转化，有用b-FGF的吗？

Yorkshining

回复 yaojing929 的帖子% ]0 ]' E6 ?0 c0 H

7 J, X2 ^3 \$ t! L2 f+ v这个诱导没关注过，所以也不太清楚，应该有不少关于这方面的文章和专利吧，你可以去查查看……