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克隆猪胚胎在桑葚胚阶段经超低温保存处理后仍然具有发育能力
/ F' ^" M: R" u) aCloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage.
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, x$ o( C, i; MNakano K, Matsunari H, Nakayama N, Ogawa B, Kurome M, Takahashi M, Matsumoto M, Murakami H, Kaji Y, Nagashima H.
9 U2 @2 q$ v# C: R" [" c! wLaboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University.6 X& {2 ~* N9 G# p
7 C: \6 h+ ]$ p- c, r/ \原文来自于http://www.jstage.jst.go.jp/arti ... 1101070340/_article
; U5 m2 i/ D. R* b3 B; R5 a* C由干细胞之家hyde翻译，转贴请注明
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摘要
: {" \" Y. ]5 ^* Z# [ P本研究的目的在于了解猪体细胞核移植（SCNT）过程中，在对移植前胚胎进行超低温保存处理后移植的整体效率情况。体细胞核移植胚在体外培养重构，与前脂肪细胞preadipocytes体外成熟的卵母细胞IVM oocytes共培养，培养到桑椹期胚胎阶段。然后使用delipation（去除细胞质中的脂滴）处理和玻璃化vitrification。在waiming（？复苏吗）和培养后，桑葚胚发育到囊胚阶段，然后移植到受体母猪中产生克隆小猪。开始对372个胚胎进行重构，有188个胚胎（50.5％）达到桑椹期，在玻璃化冷冻后有117个胚胎（31.5％）发育到胚囊期。移植98（26.3％）个形态正常的囊胚，产生了6头（1.6％）仔猪，其中包括1死胎。相比之下，没有经过冷冻保存过程的猪体细胞克隆效率是2.7%（17/635），两者是有可比性的。在研究中，我们证明了在猪体细胞克隆的过程中，在移植之前进行冷冻保存，核移植效率没有显著降低。
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& \! `% Y' b3 Q# `1 J3 F5 YAbstract; C5 A% q; R- T4 j" V& T( s) E
The aim of the present study was to clarify the overall efficiency of porcine somatic cell nuclear transfer (SCNT) by incorporating cryopreservation of the cloned embryos before transfer. The SCNT embryos reconstructed with preadipocytes and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer. and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation (2.7%, 17/635). Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer.
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个人理解，有错见谅9 Z1 R- ]3 I5 ^
3 v. A6 ]9 {) z: ?$ i2 v原文链接http://www.jstage.jst.go.jp/arti ... 1101070340/_article4 Q5 `! h) Y4 a7 d7 j
第一感觉是2.7%和1.6%差得好多…… |
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发表于 2011-1-29 14:56
