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原文来源于Pubmed 数据库，由干细胞之家新闻小组成员qianqianlaile翻译* c3 W" p: {3 f6 Z9 s: I
译文如下：9 L& s! k+ N4 \ O5 b
牙周种植学杂志 2011 2;41(1):30-43 2011-2-28 电子版
# G7 F5 w( R3 m# o" _' G, ^分析培养的人牙周膜细胞矿化期间基因的表达
+ n; C4 G3 P$ @1 p3 b# `Choi HD, Noh WC, Park JW, Lee JM, Suh JY.
6 Q8 ]7 ^% o$ t2 m. ?- Y/ x$ |2 a韩国 大丘 庆北国立大学牙科学院 牙周系
* x, H+ g$ R+ K+ J& i5 O摘要
/ q( L. U7 s8 R: p6 z目的：牙周膜干细胞在不同培养条件下能分化成类牙骨质细胞、脂肪细胞和成胶原细胞。先前的一些研究报道称牙周膜中存在的干细胞受适当刺激时参与重建牙周组织和矿化组织，使得牙周膜能有再生能力。因此本研究分析了参与人牙周膜细胞分化矿化过程的基因，并搜索了可能与人牙周膜细胞矿化相关的替代基因。 3 p- ]5 m9 o: C; J' b0 V
方法: 用或不用成骨诱导培养基培养人牙周膜细胞，在培养的第7和14天分别进行DNA微矩阵分析以研究人牙周膜细胞分化期间的基因表型。再用逆转录聚合酶链反应验证DNA微距阵数据。
7 x& U8 |2 s$ g" x2 v结果: 成骨培养基培养人牙周膜细胞7天时基因上调被认为与钙/铁/金属离子粘合或体内平衡 (PDE1A, HFE and PCDH9) 及细胞活力(PCDH9)有关，且基因下调被认为与增殖(PHGDH and PSAT1)有关。同样地, 成骨培养基培养人牙周膜细胞14天时基因上调被认为与细胞凋亡, 血管形成(ANGPTL4 and FOXO1A)及脂肪形成 (ANGPTL4 and SEC14L2)有关, 且基因下调被认为与细胞迁移 (SLC16A4)有关.
* x7 R- x _" N, |结论: 本研究说明牙周膜中干细胞在适当的刺激下能分化成成骨细胞/成牙骨质细胞，且基质成熟阶段与钙粘合相关的基因(PDE1A and PCDH9)的强表达可能与人牙周膜细胞分化成成骨细胞/成牙骨质细胞有关。2 _8 M" y: I/ t8 W7 B! R1 Q
* t- F1 `. a4 m) a- e% m原文如下：2 S' B' S( w$ v6 a, N4 V
J Periodontal Implant Sci. 2011 Feb;41(1):30-43. Epub 2011 Feb 28.; c1 j3 q5 a% V# @" J3 O& B7 t
Analysis of gene expression during mineralization of cultured human periodontal ligament cells.- U1 r4 F9 Y. u! l7 v. C2 H
Choi HD, Noh WC, Park JW, Lee JM, Suh JY./ `! |" `) Y% c D3 H/ V
Department of Periodontology, Kyungpook National University School of Dentistry, Daegu, Korea.
; d' X0 P6 ? ?5 x$ IAbstract& } n9 y% r0 b! M F1 \
PURPOSE: Under different culture conditions, periodontal ligament (PDL) stem cells are capable of differentiating into cementoblast-like cells, adipocytes, and collagen-forming cells. Several previous studies reported that because of the stem cells in the PDL, the PDL have a regenerative capacity which, when appropriately triggered, participates in restoring connective tissues and mineralized tissues. Therefore, this study analyzed the genes involved in mineralization during differentiation of human PDL (hPDL) cells, and searched for candidate genes possibly associated with the mineralization of hPDL cells.
1 \2 B7 O4 X Y A, CMETHODS: To analyze the gene expression pattern of hPDL cells during differentiation, the hPDL cells were cultured in two conditions, with or without osteogenic cocktails (β-glycerophosphate, ascorbic acid and dexamethasone), and a DNA microarray analysis of the cells cultured on days 7 and 14 was performed. Reverse transcription-polymerase chain reaction was performed to validate the DNA microarray data. q- [' a7 g. A6 c! S
RESULTS: The up-regulated genes on day 7 by hPDL cells cultured in osteogenic medium were thought to be associated with calcium/iron/metal ion binding or homeostasis (PDE1A, HFE and PCDH9) and cell viability (PCDH9), and the down-regulated genes were thought to be associated with proliferation (PHGDH and PSAT1). Also, the up-regulated genes on day 14 by hPDL cells cultured in osteogenic medium were thought to be associated with apoptosis, angiogenesis (ANGPTL4 and FOXO1A), and adipogenesis (ANGPTL4 and SEC14L2), and the down-regulated genes were thought to be associated with cell migration (SLC16A4).
. \/ @5 w1 w" ?+ QCONCLUSIONS: This study suggests that when appropriately triggered, the stem cells in the hPDL differentiate into osteoblasts/cementoblasts, and the genes related to calcium binding (PDE1A and PCDH9), which were strongly expressed at the stage of matrix maturation, may be associated with differentiation of the hPDL cells into osteoblasts/cementoblasts.3 j3 e E( N4 b1 F: b
PMID: 21394295 [PubMed - in process]PMCID: PMC3051055Free PMC Article) C. u4 r% e4 k& A$ [$ f9 M
M; l% l s' Q; x8 P注明：个人翻译，理解不准确的语句还望大家积极指出。 |
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发表于 2011-3-21 09:36
