多能干细胞基因组编辑：方法，误区和解决方案

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1月6日，Cell子刊《Cell Stem Cell》在线发表了来自哈佛大学和麻省总医院研究人员的一篇综述文章，题为“Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions”。
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/ F* d$ n8 ^- ^# zGenome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions7 S7 E, d! m4 h& s- o& j
Summary: Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.& x& ?7 g6 t$ c5 B9 Y
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addstemcell

TALEN,CRISPR-CAS9都对iPS和ES进行编辑的，就是要看最终的实验目的是什么，若治疗用的话，请采用TALEN技术，CRISPR-CAS9有脱靶效应。若一般的基础实验，仅仅验证一下功能，CRISPR-CAS9也是可以应用的。

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