日本研究人员从女性月经血中成功提取干细胞

naturalkillerce

回复 linchee 的帖子
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6 F% a, V, k9 e' h2 s不是子宫内膜肝细胞，而是间充质干细胞。

linchee

回复 wind789 的帖子' U5 y( ~  X# K4 x

( L4 H1 @" w! q* P0 A, i' P3 o  X月事杯有专利的，在收集的时候作为套装一起

linchee

回复 naturalkillerce 的帖子
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* v0 B/ z3 a: U; v8 V  M' \您觉得这个间充质干细胞是来源于哪呢？我认为是来源于子宫内膜，现在有很多名称比较混乱：menstrual blood stem cell/endometrial stem cell...当然现在这个还没有定论，有文章将这些统称为ERC（endometrial regenerative cell）

naturalkillerce

回复 linchee 的帖子
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就事论事，根据Shunichiro Miyoshi(中文名：三善俊一郎)发表在《Stem Cells》上描述是经血间充质干细胞。但是，这个间充质干细胞到底来源哪？确实是个问题，这方面还没有。要不兄弟你多看看文献多研究一下，然后给大家讲解分享一下。呵呵。

naturalkillerce

回复 linchee 的帖子
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- u! m5 f8 X! q- ]; b2 w+ H, X上传文献，分享一下，呵呵。
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; V4 ]* W2 J9 D& a+ ?  N" B$ o; sNovel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells
6 \; b6 U; ~9 oNaoko Hida, Nobuhiro Nishiyama, Shunichiro Miyoshi, Shinichiro Kira, Kaoru Segawa, Taro Uyama, Taisuke Mori, Kenji Miyado, Yukinori Ikegami, ChangHao Cui, Tohru Kiyono, Satoru Kyo, Tatsuya Shimizu, Teruo Okano, Michiie Sakamoto, Satoshi Ogawa, Akihiro Umezawa. F7 R# U2 X2 Y( b; D$ Q! I% g
STEM CELLS Volume 26, Issue 7, pages 1695–1704, July 20081 E* M+ r; F: u+ _
DOI: 10.1634/stemcells.2007-0826
" v7 t. w+ \9 n. Q, Chttp://onlinelibrary.wiley.com/d ... .2007-0826/abstract
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naturalkillerce

回复 linchee 的帖子. D) K6 ^/ m* }+ D: H
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文章中对经血间充质细胞的来源也作了判断，猜测很有可能来自子宫内膜腺(uterine endometrial gland)。由于这是2008年的文献，不知两三年来，这方面的确实消息如何？
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9 w3 _7 I' x( d; H" K5 G/ D8 }) hCell surface marker analysis revealed that MMCs are neither encirculating endothelial progenitor cells nor macrophages, but are mesenchymal phenotype cells. We speculated that MMCs may originate in uterine endometrial glands since a lot of detached endometrial glands were observed in menstrual blood and EMCs have the same surface marker as the MMCs, as well as an extremely high cardiomyogenic potential (76.4%–96.5% and 33.2%, respectively). As has been reported, MSCs cannot be detected in circulating blood and all tissues have MSC reservoirs localized in the perivascular niche, so EMCs and MMCs do not seem to originate from BMMSCs.7 r2 {2 J! n* H( L! @8 S
其中：
) A' n: _" U9 y. X( Emenstrual blood-derived mesenchymal cells (MMCs)
) z+ a$ q6 X5 F1 l9 b( t: Fendometrial gland-derived mesenchymal cells (EMCs)
5 |0 h9 ]" o0 Obone marrow-derived mesenchymal stem cells (BMMSCs)

ellapan

回复 zhanglei1414 的帖子
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( h3 O1 D; x9 M哈哈 好有趣

linchee

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! q# i3 {- I) X; V0 [回复 naturalkillerce 的帖子
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呵呵，在月经周期随着激素的撤退，子宫内膜血管收缩，内膜缺血坏死剥脱，随血流出。# k% q8 J  ^/ K. d# v
我觉得经血只是这个过程的载体，经血中含大量内膜剥脱物，细胞应该是来源于那些剥脱物吧。现在这种细胞的分离有很多方式，包括子宫的切除，刮宫，经血，我一直想弄清楚到底来源哪里。之前一篇小文章说这些是同一细胞，一老外写信反驳我，我就这样说说后来就没回音了，不知道神马情况( `0 V# f5 M( t  v' O3 O: z; e1 X
近年这个细胞的研究颇多，I型糖尿病，帕金森，中风，肢端缺血坏死。。
; A! W" e4 g9 R一个字：经血是个库。" _, H) o+ P; e6 m+ W+ a

zhumd

给大家看一下menstrual blood的采集步骤：
1 r6 b0 u1 [: n; a4 F* @2 G8 q3 Q1) The bladder is emptied prior to inserting the menstrual cup for optimum comfort during collection.3 r0 v( o) L  D- B" o& E
2) After washing the hands, the menstrual cup is rinsed with warm water and is folded by pressing the sides of the lightweight flexible cup together, as shown in the figure./ A( x8 i5 J+ d8 h
3) Then, it is folded in half again; followed by holding the cup between the thumb and index finger.
. {/ {5 L$ L6 x  }! E: @4) To insert the cup, the customers sit in a relaxed position and the folded menstrual cup is inserted completely in to the vagina. The cup would unfold by the release of the fingers.! l& @2 b4 T% A& \8 ?

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zhumd

看大家对menstrual blood这么感兴趣，我又特意将美国Cryocell公司的collection kit 找出来给大家看看，满足一下大家的好奇心。目前Cryocell的这个专利技术已经应用于市场，在中国是授权杭州一家公司在做储存，在印度是授权lifecell公司在做。* L5 W- R7 U& t

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; u, {6 K( h" |' x6 V采集套装的介绍：
+ x# Z# o8 ^6 W- ?  Q. E  L% EInside, you’ll find everything needed for you to collect and send your C’elle menstrual stem cells for processing and preservation, including a menstrual cup, collection tubes, prepaid FedEx airbill for return shipment to Cryo-Cell, and comprehensive instructions for use.
& K4 b" z* E; C% W$ U; rThe new service, called C’ellesm (pronounced “C-L”), enables women to collect menstrual flow containing stem cells, which can be cryogenically preserved in a manner similar to stem cells from umbilical cord blood and may one day serve as a potential source for promising regenerative therapies to treat heart disease, diabetes, neurological disorders like spinal cord injury, Parkinson’s and Alzheimer’s diseases, in addition to cosmeceutical applications such as anti-aging therapies, to name a few. However, realistically, it may take several years for these menstrual stem cells to be developed into potential widely-available commercial therapies. The C’elle service is based on Cryo-Cell’s intellectual property, for which patent applications are pending, related to the procurement, processing, isolation and cryo-preservation of these unique menstrual stem cells.
# I! W8 @' d) q3 ]1 `The unique C’elle service is being offered following Cryo-Cell’s discovery of new scientific evidence that menstrual flow, which results from the shedding of the uterine lining (endometrium) during menstruation, contains millions of stem cells that have many properties and characteristics similar to those of both bone marrow and embryonic stem cells. Dr. Amit N. Patel, Director of Cardiac Stem Cell Therapies at the McGowan Institute, University of Pittsburgh Medical Center, along with other independent research laboratories, studied these menstrual stem cells, which have demonstrated the capability in vitro to differentiate into neural, cardiac, bone, cartilage, and adipose cells, and possibly other cell types. Dr. Patel’s preliminary findings were presented on October 21, 2007 at TCT 2007, the annual scientific symposia of Transcatheter Cardiovascular Therapeutics, in a seminar entitled “Novel Cell Sources for Myocyte Repair and Replacement”. ( I4 D5 R; r" O& h) x6 M

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