|
- 积分
- 16
- 威望
- 16
- 包包
- 538
|
本帖最后由 细胞海洋 于 2010-1-27 09:30 编辑
( |7 e* a! v1 t1 Z$ A' D% l9 _- C+ l ?9 e# Y
breaking news in cell stem cell2 ^ Z$ l$ l( A( m
http://www.cell.com/cell-stem-cell/home8 ]7 l8 \: J1 S& ]0 d
z/ k9 j" ~$ `# n& nSomatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) with the introduction of Oct4, Sox2, Klf4, and c-Myc. Among these four factors, Oct4 is critical in inducing pluripotency because no transcription factor can substitute for Oct4, whereas Sox2, Klf4, and c-Myc can be replaced by other factors. Here we show that the orphan nuclear receptor Nr5a2 (also known as Lrh-1) can replace Oct4 in the derivation of iPSCs from mouse somatic cells, and it can also enhance reprogramming efficiency. Sumoylation mutants of Nr5a2 with enhanced transcriptional activity can further increase reprogramming efficiency. Genome-wide location analysis reveals that Nr5a2 shares many common gene targets with Sox2 and Klf4, which suggests that the transcription factor trio works in concert to mediate reprogramming. We also show that Nr5a2 works in part through activating Nanog. Together, we show that unrelated transcription factors can replace Oct4 and uncovers an exogenous Oct4-free reprogramming code.
3 G, _+ p+ q! q' u0 P$ r. f
! }. t" B( S o; \7 Z3 U3 b楼下原文 感谢lingren 提供 |
-
总评分: 威望 + 10
包包 + 10
查看全部评分
|
发表于 2010-1-26 22:35
