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回复 zerollx 的帖子9 M+ m/ |; Y5 [# O# V2 z' m7 p1 r
4 b+ C" H/ N( P好像看有的说F的意思就是fast,生长速度比293T快,它们两个都是整合了SV40大T抗原。具体的也不是很清楚。2 c) V1 c% E3 b8 t3 f% W
The 293FT Cell Line is derived from the 293F Cell Line and stably
8 o' U5 H7 _9 B0 [7 W" l& T- Iexpresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid." T' u7 L" e. I! }: i; q8 j& Z# v
Expression of the SV40 large T antigen is controlled by the human cytomegalovirus: H7 h0 k, E- T3 E$ _* N
(CMV) promoter and is high-level and constitutive. Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPower Lentiviral Expression System available from Invitrogen.# c+ v r) W; C& O
The 293 Cell Line is a permanent line established from primary embryonal human
+ w, s2 ^" r2 Z' N. rkidney transformed with sheared human adenovirus type 5 DNA (Graham et al.,, E. L4 F8 Q7 a8 m
1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells: E* n, y, @. R$ u! \4 t
and participates in transactivation of some viral promoters, allowing these cells to
* `& N9 {% z' rproduce very high levels of protein.
* ` }+ J1 s1 T: u, }! v# TThe 293-F Cell Line available from Invitrogen (Catalog no. 11625) is a fastgrowing; r+ V4 {6 ]/ D: p3 w" E
variant of the 293 cell line, and was originally obtained from Robert Horlick at Pharmacopeia.
$ @+ [) d9 V5 G- G1 n) i9 t这个以供参考! |
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