有养肝癌细胞的朋友没？筛选出的肝癌干细胞亚群药用什么培养基？谢谢了啊

renkq

有这样的文献，Cao L, Zhou Y, Zhai B, Liao J, Xu W, Zhang R, Li J, Zhang Y, Chen L, Qian H, Wu M, Yin Z. Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines. BMC Gastroenterol. 2011, 11: 71.  Department of Molecular Oncology, Second Military Medical University, Shanghai, China.  人肝癌细胞系中具有肿瘤干细胞性质的成球细胞亚群; k. A; e1 i+ R) \) U7 B
干细胞培养条件： Cells were collected and washed to remove serum, then suspended in serum-free DMEM/F12 supplemented with 100 IU/ml penicillin, 100 μg/ml streptomycin, 20 ng/ml human recombinant epidermal growth factor (hrEGF), 10 ng/ml human recombinant basic fibroblast growth factor (hrbFGF), 2% B27 supplement without vitamin A, 1% N2 supplement (Invitrogen, Carlsbad, CA, USA).

zengfang1102

谢谢