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发表于 2009-3-6 00:06 |只看该作者 |倒序浏览 |打印

Molecular NeuroPathoBiology Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom' y; p, ?4 E$ Y( f

Address correspondence to Giampietro Schiavo, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, Room 614-5, London WC2A 3PX, UK. Tel.: 44-20-7269-3300. Fax: 44-20-7269-3417. E-mail: g.schiavo@icrf.icnet.uk+ q/ w/ F+ V  r0 d" a3 Y
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Abstract

Axonal retrograde transport is essential for neuronal growth and survival. However, the nature and dynamics of the membrane compartments involved in this process are poorly characterized. To shed light on this pathway, we established an experimental system for the visualization and the quantitative study of retrograde transport in living motor neurons based on a fluorescent fragment of tetanus toxin (TeNT HC). Morphological and kinetic analysis of TeNT HC retrograde carriers reveals two major groups of organelles: round vesicles and fast tubular structures. TeNT HC carriers lack markers of the classical endocytic pathway and are not acidified during axonal transport. Importantly, TeNT HC and NGF share the same retrograde transport organelles, which are characterized by the presence of the neurotrophin receptor p75NTR. Our results provide the first direct visualization of retrograde transport in living motor neurons, and reveal a novel retrograde route that could be used both by physiological ligands (i.e., neurotrophins) and TeNT to enter the central nervous system.
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Key Words: axonal transport; motor neuron; endosome; tetanus toxin; p75NTR

Introduction$ S; }, t* O! H( {. R

Neurons are highly polarized cells characterized by a complex network of trafficking pathways, allowing communication over long distances. Anterograde transport ensures delivery of newly synthesized proteins and organelles to their peripheral destinations (Nakata et al., 1998; Goldstein and Yang, 2000; Kaether et al., 2000). Several organelles and ligands are targeted to the cell body via retrograde transport, which is essential for their biological functions (Goldstein and Yang, 2000). Examples include endogenous molecules, like neurotrophins (Goldstein and Yang, 2000), and pathogens, such as Herpes simplex virus (Bearer et al., 2000).
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Recently, much effort has been dedicated to the study of anterograde transport in different types of neurons (Morris and Hollenbeck, 1995; Nakata et al., 1998; Kaether et al., 2000). Retrograde routes were also identified in hippocampal and sympathetic neurons (Parton et al., 1992; Hollenbeck, 1993; Prekeris et al., 1999) by following recycling endosomal markers and fluid phase tracers. However, little is known about the dynamics and the principles regulating retrograde transport.0 p' @: U# Y- G# L) H( Q  ^

In this study we sought to investigate this process in living mammalian motor neurons (MNs)* using a nontoxic fragment of tetanus toxin (TeNT). TeNT and its binding fragment provide excellent probes for this purpose because they are among the best examples of ligands undergoing specific uptake and retrograde transport in neurons. TeNT belongs to the clostridial neurotoxin family and enters the nervous system at the neuromuscular junction (NMJ), where it is internalized and retrogradely transported along the MN axons by an unknown mechanism (Schiavo et al., 2000). After reaching the cell body, TeNT is transcytosed into adjacent inhibitory interneurons where it blocks neurotransmitter release, leading to spastic paralysis.

TeNT is composed of a heavy (H) and a light (L) chain linked by a disulphide bond. The L chain is responsible for intracellular activity, whereas the H chain mediates binding and internalization into neurons (Schiavo et al., 2000). The COOH-terminal 50-kD domain of TeNT (HC) is both necessary and sufficient for binding and internalization in spinal cord cells and purified MNs (Lalli et al., 1999; Herreros et al., 2000). When added to spinal cord cells and to NMJ preparations, TeNT HC prevents uptake of TeNT, leading to a block of the holotoxin effects and indicating that the HC binding is fully functional (Lalli et al., 1999). Furthermore, TeNT HC undergoes axonal retrograde transport in vivo, as shown by the accumulation of HC in the central nervous system after its intramuscular injection (Schiavo et al., 2000).; M6 Q( U9 c2 J: X5 F4 M5 v$ L/ u1 x

In this work we generated a fluorescent TeNT HC and followed its trafficking in MNs, the cells competent for TeNT endocytosis and transport in vivo. In this cellular system, TeNT HC is specifically recruited to a novel retrograde transport route, which uses carriers with distinct morphology and average speed. A large fraction of TeNT HC retrograde organelles is shared by NGF and contains the neurotrophin receptor p75 (p75NTR), suggesting that TeNT may use a physiological retrograde transport pathway to reach the soma of MNs.$ m  D4 K& z( z. `

Results and discussion* i* S' W3 y9 q- g
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Fluorescent TeNT HC can be used to monitor retrograde transport in living MNs

To create a tool to investigate retrograde transport, we expressed a recombinant TeNT HC tagged at the NH2 terminus with a domain containing four cysteines (Griffin et al., 1998), which constitutes a site for preferential modification by thiol-specific fluorescent reagents, such as Alexa Fluor 488 maleimide. The fluorescent TeNT HC Alexa488 specifically binds, and is internalized by, purified MNs in culture, a process that is prevented by preincubation with an excess of native TeNT (unpublished data).* ?% S' ^( A5 L( y! w; \4 W
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We used TeNT HC Alexa488 to follow retrograde transport in living MNs. Cells were incubated with 25–40 nM fluorescent TeNT HC for 15 min at 37°C, washed, and imaged by confocal or low-light microscopy. The plasma membrane appeared highly stained, particularly at neurite contacts and synaptic sites. Cell bodies and dendrites also displayed a bright staining pattern (Fig. 1 a), suggesting that TeNT HC binding sites are not restricted to the axonal surface in cultured MNs. An earlier study suggested that TeNT could enter hippocampal neurons through synaptic vesicle recycling (Matteoli et al., 1996). In contrast, in MNs, TeNT HC is internalized in the absence of depolarization and displays very limited colocalization with synaptic vesicle markers (unpublished data). This is in agreement with reports showing that TeNT uptake and retrograde transport are unaffected at NMJs in which neurotransmitter release has been inhibited (Schiavo et al., 2000). Therefore, the uptake mechanism of TeNT at the NMJ could be different from that occurring in central neurons.

Figure 1. Visualization of TeNT HC retrograde carriers. Cells were incubated with TeNT HC-Alexa488 for 15 min at 37°C, washed, and imaged by low-light time-lapse microscopy. (a) Low-magnification image of an MN displaying vesicular staining. (b–g) Time series imaging of the axon in the boxed area in a. Intervals between frames are 5 s. Both round vesicles (arrow) and tubular structures (arrowhead) travel toward the cell body (see Video 1, available at http://www.jcb.org/cgi/content/full/200106142/DC1). (h–j) Example of a tubular endosome bending during retrograde transport along a single axon (arrowhead). A slower round vesicle is also indicated (asterisk). The cell body is located out of view at the bottom of the picture. Bars: (a–g) 5 μm; (h–j) 2 μm.

Fluorescent carriers appeared in axons 20 min after washing the cells. This lag may be due to a maturation phase of the endocytic compartment preceding the loading on transport tracks (Ure and Campenot, 1997). Remarkably, the vast majority of TeNT HC–labeled structures moved in a retrograde fashion, whereas anterograde vesicles were extremely rare. Low-light time-lapse microscopy revealed the presence of pleiomorphic TeNT HC carriers (Fig. 1, b–g; Video 1, available at http://www.jcb.org/cgi/content/full/200106142/DC1). We observed slow round vesicles and fast tubules, which continued to move until they disappeared from the field of view. A small fraction of the vesicles were stationary or oscillating, and appeared as bright round structures scattered along axons. We also observed long tubules bending while moving, indicating that they may switch transport tracks en route (Fig. 1, h–j).- O' c- U4 [; e

Tracking of TeNT HC carriers reveals different components of retrograde transport. \( X1 S" l) s: N* a

The kinetics of retrograde transport in living MNs can be analyzed by following the displacement of the TeNT HC–labeled carriers. Different groups of structures undergoing retrograde transport can be distinguished: tubules (Fig. 2, a and b, ?), which showed a fast movement, and bright round vesicles (Fig. 2, a and c, ) with a slower speed and frequent stationary periods. Other types were rare bright oval bodies and faint round carriers (Fig. 2, a and c, ), which displayed all types of movement seen for tubules and bright vesicles. Tubules and vesicles represent the majority of TeNT HC–labeled structures (66%) (Fig. 2 e).
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Figure 2. Kinetic analysis of TeNT HC carriers in MNs. (a) Displacement of 15 carriers tracked during a representative experiment. Retrograde movement is conventionally shown as positive. Intervals between time points are 5 s. Note the presence of fast tubules (?) and slower vesicles (). Half-filled squares refer to other types of carriers. (b) Displacement graphs of the tubules from a, setting the start of tracking as time 0, show an apparently constant movement with similar speed. (c) Displacement graphs of the remaining carriers from a show a slower and more discontinuous movement. (d) Average speeds of TeNT HC retrograde carriers (; n = 256 carriers from three independent experiments). Round vesicles (; n = 76) have an average speed distribution different from tubules (?, n = 93). (e) Percentage of the different types of carriers analyzed in d.) X/ d  I: l% B# K3 b- ?; \# B

The average speed distribution of all the retrograde organelles revealed the presence of multiple components (Fig. 2 d, ). Deconvolution analysis confirmed three populations of carriers identified by Gaussian distributions with average speeds of 1.0, 1.5, and 2.1 μm/s, respectively. This trimodal representation allowed the best fitting of the observed average speed curve with lower deviation (R2 = 0.996) than the corresponding bi- or unimodal. Strikingly, this trimodal distribution reflected the speed of the round vesicles, which show a single peak at 1.0 ± 0.29 μm/s (Fig. 2 d, ), and of the faster tubules peaking at 1.5 ± 0.36 and 2.1 ± 0.13 μm/s (Fig. 2 d, ?). Other types of carriers displayed a trimodal distribution as the total frequency curve (unpublished data). In contrast, the rare carriers moving transiently in the anterograde direction presented an average speed of 0.23 μm/s.6 J, E7 j8 _  `9 s( q3 Z+ d' \
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These results demonstrate that TeNT HC uses pleiomorphic axonal carriers, which can be divided in two major morphological classes with distinct kinetic properties: round vesicles and tubulo-vesicular organelles. Retrograde round endosomes had been observed in dorsal root ganglia neurons (Nakata et al., 1998). Long tubules are used for the anterograde delivery of newly synthesized proteins to the neuronal periphery (Nakata et al., 1998; Kaether et al., 2000), whereas bidirectional tubulo-vesicular structures belong to a sorting/recycling compartment in hippocampal neurons (Prekeris et al., 1999). Our study identifies tubular structures as novel axonal carriers characterized by a fast and apparently continuous retrograde movement. The speeds observed for TeNT HC transport are in the range reported for fast axonal transport (1–5 μm/s) (Nakata et al., 1998; Goldstein and Yang, 2000; Kaether et al., 2000) and closely match those observed for TeNT in vivo (0.8–3.6 μm/s) (St?ckel et al., 1975). Strikingly, TeNT HC carriers showed a clear bias for retrograde movement, indicating a specific association with one or more types of retrograde motors.

TeNT HC retrograde carriers do not colocalize with acidic organelles and lysosomes0 E! p% z( X) J2 B2 Y  ]' L
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Conflicting results on the fate of TeNT after endocytosis have been reported. Although some suggested that TeNT might escape lysosomal degradation in vivo, others found TeNT in multivesicular bodies and lysosomes (Schiavo et al., 2000). To characterize the TeNT HC compartment, we performed two-color time-lapse microscopy in living MNs using the membrane-permeable dye Lysotracker, which stains acidic organelles and lysosomes. We observed no colocalization between TeNT HC–labeled endosomes and Lysotracker-stained vesicles (Fig. 3, a–c; Video 2, available at http://www.jcb.org/cgi/content/full/200106142/DC1). Confocal time-lapse experiments and simultaneous differential interference contrast (DIC) imaging revealed that Lysotracker was particularly concentrated in phase-contrast bright round structures (Fig. 3, d–e, *), which are likely to correspond to prelysosomal organelles (Kuznetsov et al., 1992) and were always distinct from the round or tubulo-vesicular carriers labeled by TeNT HC and undetectable by DIC (Fig. 3, d–e). The Lysotracker-positive compartment is accessible to the endocytic tracer Texas red dextran (Fig. 3, f–h). Notably, we observed organelles stained by fluorescent dextran, which were not acidic (Fig. 3 h, *).
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Figure 3. TeNT HC carriers do not colocalize with acidic organelles. MNs were incubated with TeNT HC Alexa488 and Lysotracker red DND-99 for 20 min at 37°C. Cells were then washed and imaged with low-light microscopy. The cell body is located out of view to the right. Intervals between frames are 5 s. (a) Time series showing retrograde TeNT HC–labeled endosomes (arrow and ?). (b) Corresponding frames showing Lysotracker-stained organelles (arrowheads). (c) Merged images of a and b. Note the lack of colocalization between TeNT HC and Lysotracker-stained organelles (see Video 2, available at http://www.jcb.org/cgi/content/full/200106142/DC1). (d–e) Detail from confocal observation of an axonal branch point. (d) DIC image. (e) Overlap of the green and red channels with the simultaneous DIC image. TeNT HC (green) stains tubular and round carriers (arrows), whereas Lysotracker (red) labels distinct round vesicles (* and arrowheads). An asterisk marks a phase-contrast bright round organelle positive for Lysotracker, but negative for TeNT HC. (f–h) Lysotracker-positive organelles are accessible to endocytic tracers. MNs were incubated with Texas red dextran overnight and with Lysotracker green DND-26 for 30 min at 37°C. Cells were then washed and imaged by confocal microscopy. Lysotracker-positive organelles (f) are also stained by Texas red dextran (g, arrowheads). (h) Merged image of f and g. Nonacidic organelles containing only dextran are also visible (*). Bars, 5 μm.+ v4 Q5 ]3 Z  u6 b" S+ H/ h
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A comparison of the motile behavior displayed by TeNT HC– and the Lysotracker-positive organelles further demonstrates that they are distinct compartments (Fig. 4). The speed distribution for TeNT HC carriers extends to values of >3 μm/s and is retrogradely biased, whereas the organelles stained by Lysotracker are slower and display a higher frequency of anterograde movement (Fig. 4 a). Moreover, Lysotracker vesicles changed direction more frequently compared with TeNT HC organelles, as shown by the different incidence of reversal (Fig. 4 b). These observations demonstrate that TeNT HC follows an axonal retrograde route able to bypass lysosomal targeting.; ?" ]" i: X' Z9 b! d
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Figure 4. TeNT HC carriers and Lysotracker-positive organelles show distinct motile properties. (a) Relative frequency of speed values observed between two consecutive frames (interval = 5 s) for TeNT HC carriers (n = 364 vesicles, black bars) and Lysotracker-containing organelles (n = 235 vesicles, gray bars). Retrograde movement is conventionally shown as positive. (b) The incidence of reversal (number of changes of direction per organelle) for Lysotracker-positive vesicles is 11 times higher than TeNT HC carriers.4 J, w4 S0 V, _% E9 h7 |4 c
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TeNT HC organelles might be analogous to the Rab11-containing apical recycling compartment described in polarized epithelial (Casanova et al., 1999). Rab11 displayed a ubiquitous punctate staining in MNs. However, very low colocalization with TeNT HC was observed. TeNT HC carriers did not contain the early endosome protein EEA1 (Apodaca, 2001), nor the autophagic marker Apg5 and EM analysis excluded the targeting of TeNT HC to multivesicular bodies (unpublished data). Therefore, TeNT HC might use a distinct endocytic pathways able to bypass the classical endosomal system, as suggested by the long half-life of TeNT in spinal neurons (Schiavo et al., 2000).
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Recently, alternative endocytic routes were shown for Simian Virus 40 (Pelkmans et al., 2001) and Escherichia coli (Shin et al., 2000). Both pathogens use intracellular compartments that do not belong to the endosomal–lysosomal system and which involve cholesterol and glycosphingolipid-rich caveolae. Interestingly, cholesterol-enriched rafts have recently been shown to be important for neuronal intoxication by TeNT (Herreros et al., 2001). In MNs, for at least 2 h after internalization, TeNT HC compartments are not acidic and do not correspond to lysosomes along the entire axonal length nor at branch points. It was previously proposed that a restricted area of the distal axon (50–150 μm from the growth cone) and axonal branch points are major sites of acidification in the progression from endosomes to lysosomes (Overly and Hollenbeck, 1996). Our results strongly suggest that whereas endosomes are acidified by the time they reach the proximal portion of the axon, TeNT HC carriers are not. This could have important functional consequences, as TeNT has to be protected from acidification and degradation during transport to reach the adjacent interneuron in a fully active form.& @- q# g+ Y. p( s5 \" B. h. V

TeNT HC and NGF share retrograde transport carriers

To gain insights into physiological cargoes of this endocytic compartment, we analyzed whether ligands known to undergo retrograde transport in vivo are recruited into TeNT HC carriers. NGF is retrogradely transported by newborn MNs (Yan et al., 1988) and has transport rates similar to TeNT in sensory and adrenergic neurons (St?ckel et al., 1975). In MNs, NGF-containing retrograde carriers could be detected 45 min after the end of the incubation at 37°C, consistent with previous studies that used 125I-NGF in sympathetic neurons (Ure and Campenot, 1997). We detected substantial colocalization between TeNT HC and NGF, with 72% of retrograde organelles being double labeled (n = 106 carriers; two independent experiments). These organelles always corresponded to round vesicles (Fig. 5, a–c; Video 3, available at http://www.jcb.org/cgi/content/full/200106142/DC1). In contrast, we could not observe NGF in retrograde tubules containing TeNT HC (Fig. 5, e–g), suggesting that tubules and round carriers may belong to different retrograde pathways.

Figure 5. TeNT HC retrograde carriers partially colocalize with NGF-labeled compartments. MNs were incubated with TeNT HC Alexa488 and Texas red NGF for 30 min at 37°C. Cells were then washed and imaged by time-lapse confocal microscopy. The cell body is located out of view to the right. (a) TeNT HC Alexa488 and (b) Texas red NGF colocalize in retrograde carriers (arrowhead, asterisk, arrow) (see Video 3, available at http://www.jcb.org/cgi/content/full/200106142/DC1). (c) Merged image of a and b. (d) Corresponding DIC image. TeNT HC (e) and NGF (f) are found only in round vesicles (arrowhead), whereas tubules appear to be labeled only by TeNT HC (*). (g) Merged image of e and f. (h–j) Many TeNT HC carriers colocalize with p75NTR (arrowheads). MNs were incubated with TeNT HC Alexa488 (h), fixed, immunostained for p75NTR (i), and imaged by confocal microscopy. (j) Merged image of h and i. Bars, 5 μm.

In MNs, retrograde transport of NGF has been suggested to be dependent on p75NTR (Yan et al., 1993, 1988). Strikingly, >80% of TeNT HC carriers colocalized with p75NTR in axons in absence of exogenous NGF (n = 572 organelles; two independent experiments) (Fig. 5, h–j). Therefore, p75NTR represents the first membrane marker of the retrograde endocytic pathway used by TeNT HC.
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Endogenous ligands like neurotrophins might enter a retrograde transport pathway similar to the one used by TeNT to escape degradation and to reach intact the neuronal cell body. Indeed, NGF injected intramuscularly accumulates in spinal cord MNs without being degraded (Yan et al., 1988). Similar retrograde transport rates for TeNT and NGF have been reported in adrenergic neurons in vivo (St?ckel et al., 1975), which are consistent with the speed observed in our time-lapse experiments. Although the compartment responsible for p75NTR trafficking is still unknown, recent studies highlighted its association with caveolae-like domains (Huang et al., 1999). On this basis, specialized lipid domains involved in the retrograde transport of p75NTR might play an important role in the trafficking of TeNT and other virulence factors through an endocytic route avoiding degradation.: r5 G; _$ x6 m
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Materials and methods
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Materials

Reagents were from Sigma-Aldrich unless otherwise specified. Alexa Fluor 488 maleimide, Texas red sulfonyl chloride, Texas red dextran (mol wt 3,000), Texas red–conjugated goat anti–rabbit secondary antibodies, Lysotracker red DND-99, and Lysotracker green DND-26 were from Molecular Probes. Polyclonal 9992 anti-p75NTR antiserum was a gift from Dr. M. Chao (Skirball Institute, New York University, New York, NY) (Khursigara et al., 1999).

Fluorescent labeling/ Q4 [0 g1 u! U; _
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The preparation of TeNT HC tagged to the peptide AEAAAREACCRECCAREAAAR will be described elsewhere. Labeling with Alexa Fluor 488 maleimide was performed according to the manufacturer's instructions, and gave an average 1.8 moles of dye per mole of TeNT HC.
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For NGF labeling, 100 μg of mNGF 7S (Alomone Labs) in 500 mM NaHCO3, pH 8.0, was incubated for 1 h at 23°C in the dark with 100 μg of Texas red sulfonyl chloride. Labeled NGF was then purified on a PD-10 column equilibrated with 0.2% acetic acid (Sandow et al., 2000). Texas red NGF contained 4 moles of dye per mole of protein, and could differentiate PC12 cells similarly to native NGF.$ L$ I. e% ?+ w; F) g
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Retrograde transport assay in living MNs5 L9 j" ^+ ^( F! E3 ^+ \, x! t

Rat spinal cord MNs were purified from E14 embryos and maintained in culture as described (Herreros et al., 2001). Cells were seeded on glass coverslips or 35-mm glass-bottom microwell dishes (MatTek Corporation) at a density of 40,000 cells/coverslip. After 1 wk in culture, MNs were incubated with 25–40 nM fluorescent TeNT HC in Neurobasal medium (GIBCO BRL) at 37°C for 15 min, washed three times with DME without phenol red, supplemented with 30 mM Hepes-NaOH, pH 7.3, and then placed in a humidified steel chamber maintained at 37°C. For double-labeling experiments, MNs were incubated with 40 nM TeNT HC Alexa488 and 50 nM Lysotracker red DND-99 for 20 min at 37°C. For the labeling of endocytic organelles, cells were incubated with 0.9 mg/ml Texas red dextran overnight at 37°C. Lysotracker green DND-26 (80 nM) was then added for 30 min. Cells were washed , and imaged with low-light or confocal microscopy. In another set of experiments, MNs were cooled at 4°C and incubated with 40 nM TeNT HC Alexa488 and 40 nM Texas red NGF for 20 min, washed, and then warmed to 37°C. For time-lapse low-light microscopy, images were acquired every 1–5 s with a Nikon Diaphot 200 inverted microscope equipped with a Nikon X100, 1.3 NA Plan Fluor oil-immersion objective using a Nikon FITC B-2A filter and a Hamamatsu C4742–95 Orca1 cooled charge-coupled device camera (Hamamatsu Photonic Systems) controlled by the Kinetic AQM 2000 software (Kinetic Imaging Limited). For two-color video microscopy, a double dichroic filter for simultaneous imaging of fluorescein and Texas red was used (Filter Set XF53; Omega Optical). Exposure times were between 300 and 500 ms. For time-lapse confocal microscopy, images were acquired with continuous scanning using a Zeiss LSM 510 microscope equipped with a Zeiss X63, 1.40 NA DIC Plan-Apochromat oil-immersion objective. A region of interest was simultaneously excited using the 488- and 543-nm lines of a krypton–argon and helium–neon lasers, respectively. Images were collected by averaging twice at a single focal plane and the gain/offset parameters were adjusted to prevent bleed-through between channels.% O7 x# W3 \* g6 e+ ^( Z

After the transport assay, MNs were processed for immunoflurescence as described previously (Lalli et al., 1999), limiting permeabilisation with 0.1% Triton X-100 to 15 min before blocking.; b$ y( w6 q3 u. ]( A5 S3 X

Tracking and data quantification/ ^5 s" H! a9 g) \
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The Motion Analysis software (Kinetic Imaging) was used for vesicle tracking. Only moving vesicles that could be tracked for at least three time points were considered. The distance covered by a carrier between two consecutive frames or between the initial and the final tracking points was used to determine speed and average speed, respectively. Statistical analysis was performed using SigmaPlot 2000 (v 6.0; SPSS Science). Curve fitting was obtained by using KaleidaGraph (v.3.5; Synergy Software) and Origin (v.4.0; Microcal Software). Single frame-images were processed for presentation in Adobe Photoshop?5.5./ @3 I% E0 M- U# \7 y( G4 `
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Online supplemental material

All videos were acquired as described above, processed using Adobe Premiere?, and saved as Quicktime movies, and are available at http://www.jcb.org/cgi/content/full/200106142/DC1.

Video 1 shows that TeNT HC is retrogradely transported in vesicles and tubules along an MN axon (Fig. 1 a, boxed area). Cells were incubated with TeNT HC Alexa488 for 15 min at 37°C, washed, and imaged with low-light time-lapse microscopy. The cell body is located on the left. Frames were taken every 5 s. The video consists of 95 frames played at 5 frames/s. The image is 48 μm x 15 μm.: H+ }* N& ^. u& C. ^9 A
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Video 2 shows that TeNT HC carriers do not colocalize with lysosomes or acidic organelles. Rat MNs were incubated with TeNT HC Alexa488 (green) and Lysotracker DND-99 (red) for 20 min at 37°C. Cells were washed and imaged with double-color low-light microscopy. The cell body is located to the right. Frames were taken every 5 s. The video consists of 31 frames played at 5 frames/s. The image is 57 μm x 19 μm. (See Fig. 3, a–c).
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Video 3 shows that TeNT HC carriers colocalize with Texas red NGF–labeled compartments. Rat MNs were incubated with TeNT HC Alexa488 and Texas red NGF for 30 min at 37°C. Cells were then washed and imaged by confocal microscopy. The cell body is located to the right. Frames were taken every 0.24 s. The video consists of 159 frames played at 10 frames/s. The image is 47 μm x 7 μm. (See Fig. 5, a–c).) }' p, j8 h  r  e: Z0 Q
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Footnotes
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The online version of this article contains supplemental material." }. ], u$ W  @6 W3 g
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* Abbreviations used in this paper: DIC, differential interference contrast; MN, motor neuron; NMJ, neuromuscular junction; p75NTR, neurotrophin receptor p75; TeNT, tetanus toxin.  _8 @/ a3 l% T6 P2 N; A

Acknowledgments( b, ?. F: ~+ l( g

We thank the members of the ICRF Light Microscopy Laboratory for valuable help with live imaging and tracking, and G. Charras for the mathematical analysis. We are also grateful to Drs. T. Clandinin, C. Dotti, E. Lalli, C. Montecucco D. Shima, S. Tooze, G. Warren and members of the Molecular NeuroPathoBiology Laboratory for critical reading of the manuscript.

This work was supported by the Imperial Cancer Research Fund.Revised: 28 November 2001References
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