求助: 印记基因

salomemao

回复 weiyepan 的帖子1 X0 S0 o3 s8 k5 E5 E6 F6 T

  r$ U9 ~; ]6 C谢谢，非常感谢您的回复，对我很有用。

salomemao

回复 weiyepan 的帖子# u/ {; ^. f4 s4 R1 l

; N3 U4 C& d0 L/ TFour of the six well-studied imprinted clusters in the mouse (Igf2r , Kcnq1,  Pws/As ,  Gnas) are maternally imprinted and thus gain their ICE DNA methylation imprint during oogenesis. This imprint ismaintained only on the maternal chromosome in diploid cells (Fig.
( w% W8 x' _5 r, u. w/ [1A-D). The remaining two clusters ( Igf2 ,  Dlk1 ) are paternally imprinted and gain their ICE DNA methylation imprint during spermatogenesis. This imprint is maintained only on the paternal chromosome in diploid cells.6 ~: c$ R, s7 B& ?/ e8 g
上面提到Igf2是paternally imprinted cluster, 但是我们都知道Igf2是母本印记基因。这是怎么回事呢？能帮我解答一下吗？谢谢

salomemao

回复 yuhaoze 的帖子8 x% O- k: H' |+ d2 f/ M" b& X

  x+ d3 n+ [; ^) [" iFour of the six well-studied imprinted clusters in the mouse (Igf2r , Kcnq1,  Pws/As ,  Gnas) are maternally imprinted and thus gain their ICE DNA methylation imprint during oogenesis. This imprint ismaintained only on the maternal chromosome in diploid cells (Fig.8 P& A" ]4 ^! U8 i. p8 y# ]
1A-D). The remaining two clusters ( Igf2 ,  Dlk1 ) are paternally imprinted and gain their ICE DNA methylation imprint during spermatogenesis. This imprint is maintained only on the paternal chromosome in diploid cells.
5 W3 q8 U' w$ y- }6 h5 Z% C上面提到Igf2是paternally imprinted cluster, 但是我们都知道Igf2是母本印记基因。这是怎么回事呢？能帮我解答一下吗？谢谢

weiyepan

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8 F5 _: z( N6 o  u6 c回复 yuhaoze 的帖子
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不知道我理解的对不对，请教一下：. Q0 U4 n7 h& E0 x9 P& Y* q
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这个图中的paternal gene，imprinted指的是gene promoter的甲基化还是ncRNA对基因的silence作用？2 v7 ]$ i) _4 j' Z7 U. ~7 v3 b* G
我是理解成后者所以用了knock out这词，不知道用的对不对。; V% [  A7 X- O. u7 n0 a: D+ {9 A3 Q

/ |6 B( U: S; J- n" z# h; u' j8 K- E+ H回复 salomemao 的帖子/ a8 d  I- `+ [8 ]' |1 x

yuhaoze

回复 weiyepan 的帖子/ _# Z" J" x3 d3 r) |
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印迹开关主要是基因上游甲基化

yuhaoze

回复 salomemao 的帖子7 j/ ]7 h/ ^- N1 i
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不矛盾。我给你翻译翻译，在小鼠中四个研究的比较透彻的印记基因簇是母体印迹的，也就是说 这些基因在卵子形成时期，它们的ICE就被甲基化了，这种印迹状态（即沉默状态，也就是开关的甲基化状态）只在二倍体细胞的母源染色体上维持。  P3 u6 |- P- v+ s$ q! N
剩下的你就能看懂了。
- g6 n$ R% E. U$ v我不了解Igf2，不过从weiyepan提供的图来看，这个基因是从母体提供的DNA上表达的

salomemao

回复 yuhaoze 的帖子
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' @5 t. E0 W0 `7 l' }" q. j; @  Rweiyepan提供的图是基因"Igf2r"的，什么的那段话说这个基因是母本印记的，那它是不是应该在父源染色体上表达呢？

yuhaoze

回复 salomemao 的帖子
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en
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uwmxiaoqi

LZ如果想看印记基因的survey，可以看看http://igc.otago.ac.nz/home.html网站上的总结